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Literature summary extracted from

  • Xiao, Y.; Wu, J.F.; Liu, H.; Wang, S.J.; Liu, S.J.; Zhou, N.Y.
    Characterization of genes involved in the initial reactions of 4-chloronitrobenzene degradation in Pseudomonas putida ZWL73 (2006), Appl. Microbiol. Biotechnol., 73, 166-171.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.16 expression in Escherichia coli Pseudomonas putida
5.4.4.1 expression in Escherichia coli Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.16 Pseudomonas putida Q3I3W6
-
-
1.7.1.16 Pseudomonas putida ZWL73 Q3I3W6
-
-
5.4.4.1 Pseudomonas putida
-
-
-
5.4.4.1 Pseudomonas putida ZWL73
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.16 2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida ? + 2 NADP+ + H2O recombinant protein, 43% of the activity with 4-chloronitrobenzene ?
1.7.1.16 2,4-dinitrochlorobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida ZWL73 ? + 2 NADP+ + H2O recombinant protein, 43% of the activity with 4-chloronitrobenzene ?
1.7.1.16 2,4-dinitrotoluene + 2 NADPH + 2 H+
-
 Pseudomonas putida ? + 2 NADP+ + H2O recombinant protein, 98% of the activity with 4-chloronitrobenzene ?
1.7.1.16 2,4-dinitrotoluene + 2 NADPH + 2 H+
-
 Pseudomonas putida ZWL73 ? + 2 NADP+ + H2O recombinant protein, 98% of the activity with 4-chloronitrobenzene ?
1.7.1.16 2-chloronitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida 2-chloro-N-hydroxyaniline + 2 NADP+ + H2O recombinant protein, 17% of the activity with 4-chloronitrobenzene ?
1.7.1.16 2-chloronitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida ZWL73 2-chloro-N-hydroxyaniline + 2 NADP+ + H2O recombinant protein, 17% of the activity with 4-chloronitrobenzene ?
1.7.1.16 3-chloronitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida 3-chloro-N-hydroxyaniline + 2 NADP+ + H2O recombinant protein, 84% of the activity with 4-chloronitrobenzene ?
1.7.1.16 3-chloronitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida ZWL73 3-chloro-N-hydroxyaniline + 2 NADP+ + H2O recombinant protein, 84% of the activity with 4-chloronitrobenzene ?
1.7.1.16 3-nitrophenol + 2 NADPH + 2 H+
-
 Pseudomonas putida 3-(hydroxyamino)phenol + 2 NADP+ + H2O recombinant protein, 66% of the activity with 4-chloronitrobenzene ?
1.7.1.16 4-chloronitrobenzene + 2 NADH + 2 H+
-
 Pseudomonas putida 4-chloro-N-hydroxyaniline + 2 NAD+ + H2O
-
 ?
1.7.1.16 4-chloronitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida 2-amino-5-chlorophenol + 2 NADP+ + H2O reaction in presence of hydroxylaminobenzene mutase CnbB ?
1.7.1.16 4-chloronitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida 4-chloro-N-hydroxyaniline + 2 NADP+ + H2O 2.2 mol of NADPH is oxidized per mol of 4-chloronitrobenzene ?
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida N-hydroxyaniline + 2 NADP+ + H2O recombinant protein, 61% of the activity with 4-chloronitrobenzene ?
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas putida ZWL73 N-hydroxyaniline + 2 NADP+ + H2O recombinant protein, 61% of the activity with 4-chloronitrobenzene ?
5.4.4.1 4-chloro-(hydroxyamino)benzene in presence of chloronitrobenzene nitroreductase, degradation of 4-chloronitrobenzene to 2-amino-5-chlorophenol, which is the ring-cleavage substrate in degradation of 4-chloronitrobenzene Pseudomonas putida 2-amino-5-chlorophenol
-
 ?
5.4.4.1 4-chloro-(hydroxyamino)benzene in presence of chloronitrobenzene nitroreductase, degradation of 4-chloronitrobenzene to 2-amino-5-chlorophenol, which is the ring-cleavage substrate in degradation of 4-chloronitrobenzene Pseudomonas putida ZWL73 2-amino-5-chlorophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.16 cnbA
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.16 NADH NADPH is preferred over NADH Pseudomonas putida
1.7.1.16 NADPH NADPH is preferred over NADH Pseudomonas putida