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Literature summary extracted from

  • Pfeffer, J.; Richter, S.; Nieveler, J.; Hansen, C.E.; Rhlid, R.B.; Schmid, R.D.; Rusnak, M.
    High yield expression of lipase A from Candida antarctica in the methylotrophic yeast Pichia pastoris and its purification and characterisation (2006), Appl. Microbiol. Biotechnol., 72, 931-938.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 CalA, subcloning in Escherichia coli strain DH5alpha, functional expression in Pichia pastoris strain X 33, development of a fed-batch process and a semi-continuous process, overview Moesziomyces antarcticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information kinetics Moesziomyces antarcticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 tributyrin + H2O Moesziomyces antarcticus
-
 dibutyrin + butyrate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Moesziomyces antarcticus
-
 strain DSM 70725, gene calA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant CalA 3.1fold from Pichia pastoris, by cross-flow filtration and cation exchange chromatography Moesziomyces antarcticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 2033
-
 purified recombinant enzyme Moesziomyces antarcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information CalA shows preference for short-chain triglycerides, low activity with hydrophilic esters Moesziomyces antarcticus ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Moesziomyces antarcticus dibutyrin + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 CalA
-
 Moesziomyces antarcticus
3.1.1.3 lipase A
-
 Moesziomyces antarcticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 50 70
-
 Moesziomyces antarcticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 10 70 at temperatures over 70°C, the activity decreases dramatically, the activity increases continuously at temperatures from 10°C to 50°C, temperature profile, overview Moesziomyces antarcticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
-
 Moesziomyces antarcticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 7 9 rapid decrease in activity below pH 7.0 and above pH 9.0 Moesziomyces antarcticus