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Literature summary extracted from

  • Toivari, M.H.; Ruohonen, L.; Miasnikov, A.N.; Richard, P.; Penttila, M.
    Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols (2007), Appl. Environ. Microbiol., 73, 5471-5476.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 expressed in transketolase-deficient Saccharomyces cerevisiae strain (W303/tkl1 tkl2/2C) Scheffersomyces stipitis
3.1.3.68 overexpression of DOG1 in Saccharomyces cerevisiae transketolase-deficient strain H1520 containing the integrated xylitol dehydrogenase-encoding gene (XYL2) gene increases the excretion of ribitol (by 1.6fold) and D-ribose (by 3.8fold) compared to the strain lacking DOG1 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.9 additional information expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient Saccharomyces cerevisiae strain results in an 8.5fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6fold increase in ribitol production Scheffersomyces stipitis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.68 Mg2+
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Scheffersomyces stipitis
-
-
-
3.1.3.68 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.68 additional information
-
 2-deoxy-glucose 6-phosphate (100%), D-ribulose 5-phosphate (7%), D-ribose 5-phosphate (42%), D-xylulose 5-phosphate (15%) Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.68 2-Deoxy-D-glucose 6-phosphate + H2O
-
 Saccharomyces cerevisiae 2-Deoxy-D-glucose + phosphate
-
 ?
3.1.3.68 D-ribose 5-phosphate + H2O
-
 Saccharomyces cerevisiae D-ribose + phosphate
-
 ?
3.1.3.68 D-ribulose 5-phosphate + H2O
-
 Saccharomyces cerevisiae D-ribulose + phosphate
-
 ?
3.1.3.68 D-xylulose 5-phosphate + H2O
-
 Saccharomyces cerevisiae D-xylulose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XYL2
-
 Scheffersomyces stipitis
1.1.1.9 xylitol dehydrogenase
-
 Scheffersomyces stipitis
3.1.3.68 2-deoxy-glucose 6-phosphate phosphatase
-
 Saccharomyces cerevisiae
3.1.3.68 DOG1
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.68 7
-
 assay at Saccharomyces cerevisiae