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Literature summary extracted from

  • Zhang, W.; Mullaney, E.J.; Lei, X.G.
    Adopting selected hydrogen bonding and ionic interactions from Aspergillus fumigatus phytase structure improves the thermostability of Aspergillus niger PhyA phytase (2007), Appl. Environ. Microbiol., 73, 3069-3076.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expressed in Pichia pastoris strain X33 Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.8 A58E/P65S increased activity at pH 5.5 Aspergillus niger
3.1.3.8 A58E/P65S/Q191R/T271R increased activity and improved thermostability retaining 20% greater activity after being heated at 80°C for 10 min and has a 7°C higher melting temperature than that of wild type PhyA Aspergillus niger
3.1.3.8 A58E/Q191R increased activity at pH 5.5 Aspergillus niger
3.1.3.8 A58E/Q191R/T271R increased activity at pH 5.5 Aspergillus niger
3.1.3.8 E35A no activity Aspergillus niger
3.1.3.8 E35A/R168A no activity and decreased thermostability Aspergillus niger
3.1.3.8 E35A/R168A/R248A no activity and 25% loss of thermostability Aspergillus niger
3.1.3.8 E35A/R248A no activity and decreased thermostability Aspergillus niger
3.1.3.8 R168A no activity Aspergillus niger
3.1.3.8 R168A/R248A no activity and decreased thermostability Aspergillus niger
3.1.3.8 R248A no activity Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.1064
-
 myo-inositol hexakisphosphate mutant enzyme A58E/P65S/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
3.1.3.8 0.1304
-
 myo-inositol hexakisphosphate mutant enzyme A58E/P65S, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
3.1.3.8 0.154
-
 myo-inositol hexakisphosphate mutant enzyme A58E/Q191R, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
3.1.3.8 0.1623
-
 myo-inositol hexakisphosphate mutant enzyme A58E/Q191R/T271R, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger
3.1.3.8 0.1675
-
 myo-inositol hexakisphosphate wild type enzyme, at 37°C, in 0.2 M citrate buffer, pH 5.5 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus fumigatus
-
-
-
3.1.3.8 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 nickel-nitrilotriacetic acid metal affinity chromatography, DEAE cation-exchange chromatography, and Sephadex-100 gel filtration Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 Afp
-
 Aspergillus fumigatus
3.1.3.8 PhyA
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 66.3
-
 the melting temperature of the wild type enzyme is 66.3°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 2.5
-
-
 Aspergillus niger
3.1.3.8 2.5
-
 optima at pH 2.5 and 5.5 Aspergillus fumigatus
3.1.3.8 5.5
-
-
 Aspergillus niger
3.1.3.8 5.5
-
 optima at pH 2.5 and 5.5 Aspergillus fumigatus