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Literature summary extracted from

  • Kim, T.; Mullaney, E.J.; Porres, J.M.; Roneker, K.R.; Crowe, S.; Rice, S.; Ko, T.; Ullah, A.H.; Daly, C.B.; Welch, R.; Lei, X.G.
    Shifting the pH profile of Aspergillus niger PhyA phytase to match the stomach pH enhances its effectiveness as an animal feed additive (2006), Appl. Environ. Microbiol., 72, 4397-4403.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.26 mutants expressed in Pichia pastoris Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.26 D262H increased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 E228K shows a shift of the pH optimum to 3.8 and 266% greater hydrolysis of soy phytate at pH 3.5 than the wild type enzyme Aspergillus niger
3.1.3.26 E228K/K300R/K301E shows large increase in the phytase activity ratio at pH 3.5 over pH 5.5 Aspergillus niger
3.1.3.26 E228Q decreased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 K300E/K301E greater improvement in relative activity at pH 3.5 and lower specific activity than the wild type enzyme Aspergillus niger
3.1.3.26 K301E increased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 K91A decreased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 K91E increased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 K94E decreased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 K94E/K300E/K301E greater improvement in relative activity at pH 3.5 and lower specific activity than the wild type enzyme Aspergillus niger
3.1.3.26 Q50L increased activity compared to the wild type enzyme Aspergillus niger
3.1.3.26 Q50P increased activity compared to the wild type enzyme Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.0386
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger
3.1.3.26 0.1773
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger
3.1.3.26 0.2278
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
3.1.3.26 0.3219
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 ultrafiltration, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 D-fructose 6-phosphate + H2O
-
 Aspergillus niger D-fructose + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O
-
 Aspergillus niger p-nitrophenol + phosphate
-
 ?
3.1.3.26 phosphoenolpyruvate + H2O
-
 Aspergillus niger pyruvate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 6-phytase
-
 Aspergillus niger
3.1.3.26 PhyA phytase
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.26 0.52 0.7 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
3.1.3.26 5.7
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
3.1.3.26 43.97
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
3.1.3.26 44.83
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger
3.1.3.26 80.74
-
 myo-inositol hexakisphosphate mutant enzyme E228K, in 0.2 M glycine-HCl buffer at pH 3.5 and 37°C Aspergillus niger
3.1.3.26 123.1
-
 myo-inositol hexakisphosphate wild type enzyme, in 0.2 M citrate buffer at pH 5.5 and 37°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 2.5
-
 shows a bihump two-pH-optima profile at pH 5.5 and 2.5 Aspergillus niger
3.1.3.26 5.5
-
 shows a bihump two-pH-optima profile at pH 5.5 and 2.5 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.26 3.5
-
 lowest relative activity at pH 3.5 Aspergillus niger