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Literature summary extracted from
- Purification and characterization of a hyperthermostable and high maltogenic alpha-amylase of an extreme thermophile Geobacillus thermoleovorans (2007), Appl. Biochem. Biotechnol., 142, 179-193.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.1 | polyethylene glycol 8000 and Tween 20, 40, and 80 stabilized the enzyme activity | Geobacillus thermoleovorans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Benzene | 10%, 40% inhibition | Geobacillus thermoleovorans |  |
| 3.2.1.1 | butanol | 10%, 80% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | Dextran | 1%, 20% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | dithiothreitol | 1 mM, 50% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | EDTA | 1 mM, 36% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | ethanol | 10%, 36% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | glycerol | 1%, 26% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | glycine | 2%, 20% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | Hg2+ | 1 mM, complete inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | iodoacetate | 1 mM, 60% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | methanol | 20%, 45% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | N-ethylmaleimide | 1 mM, complete inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | Pb2+ | 5 mM, 80% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | phenyl methyl sulfonyl fluoride | 1 mM, 70% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | Sn2+ | 1 mM, complete inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | Sodium dodecyl sulfate | 0.2%, 58% inhibition | Geobacillus thermoleovorans | |
| 3.2.1.1 | Triton X-100 | 0.2%, 40% inhibition | Geobacillus thermoleovorans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | KM for soluble starch is 1.1 mg/ml, KM-value for potato starch or ice starch is 0.9 mg/ml | Geobacillus thermoleovorans |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ba2+ | 1 mM, 2.24fold activation | Geobacillus thermoleovorans | |
| 3.2.1.1 | Co2+ | 1 mM, 3.06fold activation | Geobacillus thermoleovorans | |
| 3.2.1.1 | Fe2+ | 1 mM, 2.72fold activation | Geobacillus thermoleovorans | |
| 3.2.1.1 | Mg2+ | 1 mM, 1.72fold activation | Geobacillus thermoleovorans | |
| 3.2.1.1 | Mn2+ | 1 mM, 1.34fold activation | Geobacillus thermoleovorans | |
| 3.2.1.1 | Ni2+ | 1 mM, 1.23fold activation | Geobacillus thermoleovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 26000 | - |
gel filtration | Geobacillus thermoleovorans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Geobacillus thermoleovorans | - |
- |
- |
| 3.2.1.1 | Geobacillus thermoleovorans NP54 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | - |
Geobacillus thermoleovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
450 U/mg. One saccharogenic alpha-amylase unit is defined as the amount of enzyme required for the liberation of 0.001 mM of reducing sugars as maltose per milliliter per minute | Geobacillus thermoleovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | alpha-cyclodextrin + H2O | 9.2% of the activity with soluble starch | Geobacillus thermoleovorans | ? | - |
? | |
| 3.2.1.1 | alpha-cyclodextrin + H2O | 9.2% of the activity with soluble starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
| 3.2.1.1 | amylopectin + H2O | 66.1% of the activity with soluble starch | Geobacillus thermoleovorans | ? | - |
? | |
| 3.2.1.1 | amylopectin + H2O | 66.1% of the activity with soluble starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
| 3.2.1.1 | pullulan + H2O | 41.1% of the activity with soluble starch | Geobacillus thermoleovorans | ? | - |
? | |
| 3.2.1.1 | pullulan + H2O | 41.1% of the activity with soluble starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Geobacillus thermoleovorans NP54 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | rice starch, starch of pearl millet, corn starch, potato starch, wheat starch | Geobacillus thermoleovorans | ? | - |
? | |
| 3.2.1.1 | starch + H2O | rice starch, starch of pearl millet, corn starch, potato starch, wheat starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 100 | - |
- |
Geobacillus thermoleovorans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 70 | 110 | 70°C: aboutb 50% of maximal activity, 110°C: about 90% of maximal activity | Geobacillus thermoleovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 100 | - |
t1/2 increases from 3.6 h to 5.6 h in presence of cholic acid | Geobacillus thermoleovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | 98.3 | - |
soluble starch | - |
Geobacillus thermoleovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 8 | - |
- |
Geobacillus thermoleovorans |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.1 | Geobacillus thermoleovorans | isoelectric focusing, pH-gradient: 3-10 | - |
5.4 |
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