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Literature summary extracted from

  • Rupesh, K.R.; Padma, K.R.; Saravanan, P.; Jayachandran, S.
    Cloning, expression, and sequence analysis of diaminopropionate ammonia lyase gene from a nonvirulent Salmonella typhimurium PU011 (2007), Appl. Biochem. Biotechnol., 141, 161-174.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.15 medicine nonvirulent Salmonella typhimurium PU011 can be used for the production of the enzyme to detoxify the neurotoxin 3-oxalyl and 2,3-dioxalyl diaminopropionate Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.15 expression in Escherichia coli PU018 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.15 0.74
-
 2,3-Diaminopropionate wild type enzyme, 0.680 using Lathyrus sativus seed extracts as substrate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 0.741
-
 2,3-Diaminopropionate recombinant enzyme, 0.683 using Lathyrus sativus seed extracts as substrate Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.15 43000
-
 SDS-PAGE of wild type and recombinant protein Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.15 Salmonella enterica subsp. enterica serovar Typhimurium
-
 PU011
-
4.3.1.15 Salmonella enterica subsp. enterica serovar Typhimurium PU011
-
 PU011
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.15 0.346
-
 of the recombinant enzyme in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 0.351
-
 of the wild type enzyme in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.15 2,3-diaminopropanoate + H2O precursor of a neurotoxin in Lathyrus sativus seed extracts Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.3.1.15 2,3-diaminopropanoate + H2O precursor of a neurotoxin in Lathyrus sativus seed extracts Salmonella enterica subsp. enterica serovar Typhimurium PU011 pyruvate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.1.15 DAPAL
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.15 diaminopropionate ammonia-lyase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.15
-
 45
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.15
-
 45
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.15 8
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.15 6 10
-
 Salmonella enterica subsp. enterica serovar Typhimurium