Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aminzadeh, S.; Naderi-Manesh, H.; Khajeh, K.; Naderi-Manesh, M.
    Purification, characterization, kinetic properties, and thermal behavior of extracellular polygalacturonase produced by filamentous fungus Tetracoccosporium sp (2006), Appl. Biochem. Biotechnol., 135, 193-208.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.15 EDTA
-
 Tetracoccosporium sp.
3.2.1.15 Triton X-100
-
 Tetracoccosporium sp.
3.2.1.15 Tween-20
-
 Tetracoccosporium sp.
3.2.1.15 Tween-80
-
 Tetracoccosporium sp.

Application

EC Number Application Comment Organism
3.2.1.15 food industry the polygalacturonase has a remarkable heat-tolerance, which makes it very attractive for industrial applications Tetracoccosporium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Al3+
-
 Tetracoccosporium sp.
3.2.1.15 Ba2+
-
 Tetracoccosporium sp.
3.2.1.15 Ca2+
-
 Tetracoccosporium sp.
3.2.1.15 Fe2+
-
 Tetracoccosporium sp.
3.2.1.15 Fe3+
-
 Tetracoccosporium sp.
3.2.1.15 Mg2+
-
 Tetracoccosporium sp.
3.2.1.15 Mn2+
-
 Tetracoccosporium sp.
3.2.1.15 additional information not inhibited by iodoacetamide and iodoacetic acid at a concentration of 1 mM Tetracoccosporium sp.
3.2.1.15 Ni2+
-
 Tetracoccosporium sp.
3.2.1.15 SDS
-
 Tetracoccosporium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Ag+
-
 Tetracoccosporium sp.
3.2.1.15 Co2+
-
 Tetracoccosporium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 36000
-
 x * 36000, SDS-PAGE Tetracoccosporium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Tetracoccosporium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 by ammonium sulfate precipitation and two-step anion-exchange chromatography Tetracoccosporium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 6.17
-
-
 Tetracoccosporium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 polygalacturonic acid + H2O
-
 Tetracoccosporium sp. galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 36000, SDS-PAGE Tetracoccosporium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 40
-
-
 Tetracoccosporium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 20 70
-
 Tetracoccosporium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 60
-
 halflife is 63.01 min Tetracoccosporium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 1.894
-
 polygalacturonic acid
-
 Tetracoccosporium sp.
3.2.1.15 2.94
-
 polygalacturonic acid
-
 Tetracoccosporium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.3
-
-
 Tetracoccosporium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3 8
-
 Tetracoccosporium sp.