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Literature summary extracted from

  • Singh, S.; Singhal, R.; Malhotra, B.D.
    Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor (2007), Anal. Chim. Acta, 582, 335-343.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.6 analysis cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) can be immobilized onto tetraethylorthosilicate derived sol-gel films. These tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films can be used for the estimation of cholesterol oleate up to 780 mg/dl (mM). These TEOS sol-gel enzyme films provide selectivity towards glucose, lactate, uric acid and ascorbic acid Pseudomonas sp.
3.1.1.13 analysis covalent immobilization of cholesterol oxidase and cholesterol esterase onto tetraethylorthosilicate sol-gel films for use as cholesterol biosensor. Films show a thermal stability up to 55°C, response time as 180 s, linearity up to 780 mg/dl, shelf life of 1 month, and detection limit of 12 mg/dl cholesterol Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pseudomonas sp.
-
-
-
3.1.1.13 Pseudomonas fluorescens
-
 commercial product
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Pseudomonas sp. cholest-4-en-3-one + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 ChOx
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 55
-
 tetraethylorthosilicate sol-gel/ChEt/ChOx (cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) immobilized onto tetraethylorthosilicate derived sol-gel film) reveal thermal stability up to 55°C Pseudomonas sp.