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Literature summary extracted from

  • Maraite, A.; Schmidt, T.; Ansoerge-Schumacher, M.B.; Brzozowski, A.M.; Grogan, G.
    Structure of the ThDP-dependent enzyme benzaldehyde lyase refined to 1.65 A resolution (2007), Acta Crystallogr. Sect. F, F63, 546-548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 expressed in Escherichia coli BL21 (DE3) cells Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.38 in 0.1 M Tris-HCl pH 7.5, 0.2 M potassium isothiocyanate, 10% (w/v) polyethylene glycol 1000, and 10% polyethylene glycol 8000 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+ four molecules are located within the protein Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
 biovar I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.38 nickel-affinity HiTrap agarose column chromatography and Sephadex S-200 gel filtration Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 benzaldehyde + benzaldehyde
-
 Pseudomonas fluorescens benzoin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate dependent, four molecules are located within the protein Pseudomonas fluorescens