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Literature summary extracted from

  • Beuth, B.; Niefind, K.; Schomburg, D.
    Crystallization and preliminary crystallographic analysis of creatininase from Pseudomonas putida (2002), Acta Crystallogr. Sect. D, 58, 1356-1358.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.10 expressed in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.10 sitting drop vapour diffusion method with 22% ethanol, 20% PEG 8000, and 0.1 M MOPS pH 7.5 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.10 28399
-
 6 * 28399, calculated from amino acid sequence Pseudomonas putida
3.5.2.10 28400
-
 6 * 28400, MALDI-TOF mass spectrometry Pseudomonas putida
3.5.2.10 170400
-
 SDS-PAGE Pseudomonas putida
3.5.2.10 173000
-
 dynamic light scattering Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.10 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.10 creatinine + H2O
-
 Pseudomonas putida creatine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.10 homohexamer 6 * 28399, calculated from amino acid sequence Pseudomonas putida
3.5.2.10 homohexamer 6 * 28400, MALDI-TOF mass spectrometry Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.5.2.10 CrnA
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.10 6 9 the enzyme tends to precipitate at pH values of under 6.0 and over 9.0 corresponding to the stability range of the enzyme Pseudomonas putida