EC Number | Application | Comment | Organism |
---|---|---|---|
2.2.1.6 | drug development | AHAS is the target of the sulfonylurea and imidazolinone herbicides | Escherichia coli |
2.2.1.6 | drug development | AHAS is the target of the sulfonylurea and imidazolinone herbicides | Saccharomyces cerevisiae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.2.1.6 | crystal structure analysis | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.2.1.6 | additional information | isozyme AHAS II mutants of residues Phe109, Met250, Arg276 and Trp464 are nearly inactive in (S)-2-acetolactate formation, but show increased activity with pyruvate and benzaldehyde compared to the wild-type isozyme | Escherichia coli |
2.2.1.6 | W464L | the mutant of isozyme AHAS II has lost the preference for 2-ketobutyrate as second substrate | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | branched-chain amino acids | feedback inhibition, differential inhibition of isozymes, overview | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.6 | pyruvate | Escherichia coli | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | (S)-2-acetolactate + CO2 | - |
? | |
2.2.1.6 | pyruvate | Saccharomyces cerevisiae | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | (S)-2-acetolactate + CO2 | - |
? | |
2.2.1.6 | pyruvate + 2-oxobutyrate | Escherichia coli | stereospecific reaction | (S)-acetohydroxybutyrate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.2.1.6 | Escherichia coli | - |
isozymes I-III | - |
2.2.1.6 | Saccharomyces cerevisiae | P07342 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.2.1.6 | 2 pyruvate = 2-acetolactate + CO2 | catalytic mechanism and active site structure, covalent intermediates | Saccharomyces cerevisiae | |
2.2.1.6 | 2 pyruvate = 2-acetolactate + CO2 | catalytic mechanism, covalent intermediates | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.2.1.6 | additional information | substrate specificity ratios of isozymes I-III, substrate recognition mechanism, overview | Escherichia coli | ? | - |
? | |
2.2.1.6 | pyruvate | stereospecific reaction | Escherichia coli | (S)-2-acetolactate + CO2 | - |
? | |
2.2.1.6 | pyruvate | stereospecific reaction | Saccharomyces cerevisiae | (S)-2-acetolactate + CO2 | - |
? | |
2.2.1.6 | pyruvate | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | Escherichia coli | (S)-2-acetolactate + CO2 | - |
? | |
2.2.1.6 | pyruvate | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | Saccharomyces cerevisiae | (S)-2-acetolactate + CO2 | - |
? | |
2.2.1.6 | pyruvate + 2-oxobutyrate | stereospecific reaction | Escherichia coli | (S)-acetohydroxybutyrate + CO2 | - |
? | |
2.2.1.6 | pyruvate + benzaldehyde | stereospecific reaction, benzaldehyde is an artificial substrate, especially of mutants of isozyme AHAS II residues Phe109, Met250, Arg276 and Trp464 | Escherichia coli | (R)-phenylacetylcarbinol + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.2.1.6 | acetohydroxy acid synthase | - |
Escherichia coli |
2.2.1.6 | acetohydroxy acid synthase | - |
Saccharomyces cerevisiae |
2.2.1.6 | AHAS | - |
Escherichia coli |
2.2.1.6 | AHAS | - |
Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | FAD | - |
Escherichia coli | |
2.2.1.6 | FAD | - |
Saccharomyces cerevisiae | |
2.2.1.6 | thiamine diphosphate | - |
Escherichia coli | |
2.2.1.6 | thiamine diphosphate | - |
Saccharomyces cerevisiae |