Literature summary extracted from
Liao, D.I.; Calabrese, J.C.; Wawrzak, Z.; Viitanen, P.V.; Jordan, D.B.
Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis (2001), Structure, 9, 11-18.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.99.12 |
purified recombinant enzyme, hanging drop vapour diffusion method at room temperature, 4-5 days, 0.0022 ml of protein solution containing 24 mg/ml protein in 50 mM Tris-HCl pH 7.5, is mixed with 0.0007 ml of precipitating well solution containing 3 M CsCl, 3 M Cs-formate, 20 mM Bis-Tris-propane-NaOH, pH 6.9, or 6 M sodium formate, 25 mM HEPES-NaOH, pH 7.0, labeling with 1.5 mM Au(CN)2, X-ray diffraction structure determination and analysis at 1.4-2.4 A resolution, multiwavelength anomalous diffraction |
Escherichia coli |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.99.12 |
Mg2+ |
required for activity |
Escherichia coli |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.1.99.12 |
D-ribulose 5-phosphate |
Escherichia coli |
the enzyme is involved in the pathway of riboflavin biosynthesis, overview |
formate + L-3,4-dihydroxy-2-butanone-4-phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.99.12 |
Escherichia coli |
P0A7J0 |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.1.99.12 |
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate |
catalytic reaction mechanism, substrate recognition and active site structure, active site consists of three glutamates, two aspartates, two histidines, and a cysteine which may provide the means for general acid and base catalysis and for coordinating the Mg2+ cofactor within the active site |
Escherichia coli |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.99.12 |
D-ribulose 5-phosphate |
- |
Escherichia coli |
formate + L-3,4-dihydroxy-2-butanone-4-phosphate |
- |
? |
|
4.1.99.12 |
D-ribulose 5-phosphate |
the enzyme is involved in the pathway of riboflavin biosynthesis, overview |
Escherichia coli |
formate + L-3,4-dihydroxy-2-butanone-4-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.99.12 |
More |
structure determination and analysis of functions of single residues, overview, dimer interface structure, localization of the active site, sequence and structure comparison |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.99.12 |
3,4-dihydroxy-2-butanone 4-phosphate synthase |
- |
Escherichia coli |