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Literature summary extracted from

  • Hong, B.S.; Yun, M.K.; Zhang, Y.M.; Chohnan, S.; Rock, C.O.; White, S.W.; Jackowski, S.; Park, H.W.; Leonardi, R.
    Prokaryotic type II and type III pantothenate kinases: The same monomer fold creates dimers with distinct catalytic properties (2006), Structure, 14, 1251-1261.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33
-
 Staphylococcus aureus
2.7.1.33
-
 Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 hanging drop vapour diffusion method using 28% (w/v) PEG 2000 mM and 0.1M Bis-Tris (pH 6.5), at 18°C Pseudomonas aeruginosa
2.7.1.33 sitting drop vapour diffusion method using 28% (w/v) PEG 2 M and 0.1 M Bis-Tris (pH 6.5), at 18°C Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.33 D101A reduced enzymatic activity Pseudomonas aeruginosa
2.7.1.33 D121A reduced enzymatic activity Pseudomonas aeruginosa
2.7.1.33 D6A reduced enzymatic activity Staphylococcus aureus
2.7.1.33 E70A reduced enzymatic activity Staphylococcus aureus
2.7.1.33 H156A slightly increased enzymatic activity Pseudomonas aeruginosa
2.7.1.33 K13A reduced enzymatic activity Staphylococcus aureus
2.7.1.33 K13A reduced enzymatic activity Pseudomonas aeruginosa
2.7.1.33 L11A reduced enzymatic activity Staphylococcus aureus
2.7.1.33 L263P reduced enzymatic activity Staphylococcus aureus
2.7.1.33 N9G strongly reduced enzymatic activity Pseudomonas aeruginosa
2.7.1.33 T10A reduced enzymatic activity Staphylococcus aureus
2.7.1.33 T157A reduced enzymatic activity Pseudomonas aeruginosa
2.7.1.33 Y137A reduced enzymatic activity Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 K+ 0.06-0.12 mM Staphylococcus aureus
2.7.1.33 K+ 0.06-0.12 mM Pseudomonas aeruginosa
2.7.1.33 Mg2+ required Staphylococcus aureus
2.7.1.33 Mg2+ required Pseudomonas aeruginosa
2.7.1.33 NH4+ induces a 5fold higher activation than either K+ or Rb+ Staphylococcus aureus
2.7.1.33 NH4+ induces a 5fold higher activation than either K+ or Rb+ Pseudomonas aeruginosa
2.7.1.33 Rb+
-
 Staphylococcus aureus
2.7.1.33 Rb+
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Pseudomonas aeruginosa Q9HWC1
-
-
2.7.1.33 Staphylococcus aureus Q6G7I0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33
-
 Staphylococcus aureus
2.7.1.33
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Pseudomonas aeruginosa ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 dimer x-ray crystallography Staphylococcus aureus
2.7.1.33 dimer x-ray crystallography Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 CoaA
-
 Staphylococcus aureus
2.7.1.33 CoaA
-
 Pseudomonas aeruginosa
2.7.1.33 type II pantothenate kinase
-
 Staphylococcus aureus
2.7.1.33 type III pantothenate kinase
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.33 ATP
-
 Staphylococcus aureus
2.7.1.33 ATP
-
 Pseudomonas aeruginosa