EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.2.2 | biotechnology | the demonstrated application of a membrane bioreactor will be a useful method for large-scale dynamic kinetic resulution (DKR) of mandelic acid and for possible other bioconversions in organic media | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.2.2 | expressed ind Escherichia coli | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.548 | - |
(S)-Mandelate | - |
Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.2.2 | 42000 | - |
SDS-PAGE | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.2.2 | Pseudomonas putida | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.1.2.2 | (S)-mandelate = (R)-mandelate | one pot enantioconvergent synthesis of R-mandelic acid ester from racemic mandelic acid is achieved by a novel aqueous/organic two-phase system with two-enzyme process consisting of (1) lipase-catalyzed enantioselective esterification of racemic mandelic acid in organic solvent and (2) in situ racemization of unreacted isomer, S-mandelic acid in aqueous phase by recombinant mandelate racemase | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.2.2 | (S)-mandelate | - |
Pseudomonas putida | (R)-mandelate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.2.2 | mandelate racemase | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 650 | - |
(S)-Mandelate | - |
Pseudomonas putida |