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Literature summary extracted from

  • Wengelnik, K.; Vial, H.J.
    Characterisation of the phosphatidylinositol synthase gene of Plasmodium species (2007), Res. Microbiol., 158, 51-59.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.11
-
Plasmodium falciparum
2.7.8.11
-
Plasmodium knowlesi
2.7.8.11 highly spliced gene with 9 exons, cDNA encodes 207 amino acids, from mRNA in pCRII-Blunt-TOPO for cloning and sequencing (transformation of Escherichia coli TOP10 and DH5alpha) Plasmodium knowlesi
2.7.8.11 highly spliced gene with 9 exons, cDNA encodes 209 amino acids, from total RNA in pCR-BluntII-Topo for cloning and sequencing (transformation of Escherichia coli TOP10 and DH5alpha), in p413 for complementation studies in Saccharomyces cerevisiae Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.11 Hy3 hybrid construct of 57 N-terminal amino acids from Saccharomyces cerevisiae PIS and 159 C-terminal amino acids from PfPIS, no in vitro activity, 60% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%) Plasmodium falciparum
2.7.8.11 Hy4 hybrid construct of 14 N-terminal amino acids from Saccharomyces cerevisiae PIS and 202 C-terminal amino acids from PfPIS, 37% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%) Plasmodium falciparum
2.7.8.11 additional information gene successfully complements a Saccharomyces cerevisiae PIS1 deletion mutant Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.11 membrane
-
Plasmodium falciparum 16020
-
2.7.8.11 membrane
-
Plasmodium knowlesi 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.11 23735
-
x * 23735, calculated Plasmodium knowlesi
2.7.8.11 23740
-
theoretical Plasmodium knowlesi
2.7.8.11 23920
-
theoretical Plasmodium falciparum
2.7.8.11 23924
-
x * 23924, calculated Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.11 Plasmodium falciparum C0H5B7
-
-
2.7.8.11 Plasmodium falciparum Q2MJB9
-
-
2.7.8.11 Plasmodium knowlesi Q2MJB8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.11 additional information
-
1.5 nmol/h/1x10(10) cells, wild-type, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression Plasmodium falciparum
2.7.8.11 additional information
-
2.0 nmol/h/1x10(10) cells, hybrid Hy4, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression Plasmodium falciparum
2.7.8.11 additional information
-
3.4 nmol/min per 1x10(10) infected red blood cells Plasmodium falciparum
2.7.8.11 additional information
-
3.7 nmol/h/1x10(10) cells, hybrid Hy3, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression Plasmodium falciparum
2.7.8.11 additional information
-
no detectable activity for hybrid Hy3, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression Plasmodium falciparum
2.7.8.11 0.000023
-
wild-type, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression Plasmodium falciparum
2.7.8.11 0.000036
-
hybrid Hy4, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol parasite membrane extracts, infected red blood cells Plasmodium falciparum CMP + phosphatidyl-myo-inositol
-
?
2.7.8.11 additional information partial complementation of PIS activity by overexpression of PfPIS/ScPIS hybrids in ScPIS1-deficient Saccharomyces cerevisiae mutant strain BY4743, analysis by enzyme assay (30°C, yeast membrane pellet, in presence of 18 mM octyl-beta-D-glucopyranoside) or lipid incorporation and extraction Plasmodium falciparum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.8.11 ? x * 23735, calculated Plasmodium knowlesi
2.7.8.11 ? x * 23924, calculated Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.7.8.11 CDP-diacylglacerol: myo-inositol 3-phosphatidyl-transferase
-
Plasmodium knowlesi
2.7.8.11 CDP-diacylglacerol: myo-inositol 3-phosphatidyl-transferase
-
Plasmodium falciparum
2.7.8.11 PfPIS
-
Plasmodium falciparum
2.7.8.11 phosphatidyinositol synthase
-
Plasmodium knowlesi
2.7.8.11 phosphatidyinositol synthase
-
Plasmodium falciparum
2.7.8.11 PI synthase
-
Plasmodium knowlesi
2.7.8.11 PI synthase
-
Plasmodium falciparum
2.7.8.11 PIS
-
Plasmodium knowlesi
2.7.8.11 PIS
-
Plasmodium falciparum
2.7.8.11 PkPIS
-
Plasmodium knowlesi