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Literature summary extracted from

  • Kim, Y.H.; Cho, K.; Yun, S.H.; Kim, J.Y.; Kwon, K.H.; Yoo, J.S.; Kim, S.I.
    Analysis of aromatic catabolic pathways in Pseudomonas putida KT 2440 using a combined proteomic approach: 2-DE/MS and cleavable isotope-coded affinity tag analysis (2006), Proteomics, 6, 1301-1318.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.5 48000
-
 SDS-PAGE Pseudomonas putida
1.14.12.10 51500
-
 alpha-subunit BenA, calculated from amino acid sequence Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 additional information Pseudomonas putida catechol 1,2-dioxygenase is induced by benzoate ?
-
 ?
1.13.11.1 additional information Pseudomonas putida KT 2440 catechol 1,2-dioxygenase is induced by benzoate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Pseudomonas putida
-
 KT 2440
-
1.13.11.1 Pseudomonas putida KT 2440
-
 KT 2440
-
1.13.11.5 Pseudomonas putida
-
-
-
1.13.11.5 Pseudomonas putida KT 2440
-
-
-
1.14.12.10 Pseudomonas putida
-
-
-
1.14.12.10 Pseudomonas putida KT 2440
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.5 Superdex gel filtration and Q-Sepharose column chromatography Pseudomonas putida
1.14.12.10 Q-Sepharose column chromatography and Superdex 200 GPC column gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 additional information catechol 1,2-dioxygenase is induced by benzoate Pseudomonas putida ?
-
 ?
1.13.11.1 additional information catechol 1,2-dioxygenase is induced by benzoate Pseudomonas putida KT 2440 ?
-
 ?
1.13.11.5 homogentisate + O2
-
 Pseudomonas putida 4-maleylacetoacetate
-
 ir
1.13.11.5 homogentisate + O2
-
 Pseudomonas putida KT 2440 4-maleylacetoacetate
-
 ir
1.14.12.10 benzoate + O2 + NADH + H+
-
 Pseudomonas putida 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?
1.14.12.10 benzoate + O2 + NADH + H+
-
 Pseudomonas putida KT 2440 2-hydro-1,2-dihydroxybenzoate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.5 HmgA
-
 Pseudomonas putida
1.14.12.10 BenA
-
 Pseudomonas putida
1.14.12.10 benzoate dioxygenase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.10 NADH
-
 Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.5 Pseudomonas putida calculated value
-
 5.74
1.14.12.10 Pseudomonas putida alpha-subunit BenA, calculated from amino acid sequence
-
 5.54