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Literature summary extracted from

  • Lu, P.K.; Tsai, J.Y.; Chien, H.Y.; Huang, H.; Chu, C.H.; Sun, Y.J.
    Crystal structure of Helicobacter pylori spermidine synthase: a Rossmann-like fold with a distinct active site (2007), Proteins, 67, 743-754.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 gene speE, sequence comparison, overexpression of the N-terminally His6-tagged enzyme in Escherichia coli strain SG13009 and in strain B834 for the selenomethionine-labeled enzyme Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.16 purified recombinant His6-tagged enzyme, also as selenomethionine-labeled enzyme, the latter from 0.1 M sodium HEPES, pH 7.5, with 33% PEG 400, two crystals Forms A and B, belonging to the monoclinic space group P21and orthorhombic space group C2221, are grown under different conditions, X-ray diffraction structure determination and analysis at 2.0-2.5 A resolution, multiwavelength anomalous dispersion Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 58000
-
dimeric enzyme form A, analytical ultracentrifugation Helicobacter pylori
2.5.1.16 60000
-
dimeric enzyme form A, gel filtration Helicobacter pylori
2.5.1.16 123000
-
tetrameric enzyme form B, analytical ultracentrifugation Helicobacter pylori
2.5.1.16 158000
-
tetrameric enzyme form B, gel filtration Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosylmethioninamine + putrescine Helicobacter pylori PAPTs are essential for bacterial cell viability 5'-S-methyl-5'-thioadenosine + spermidine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Helicobacter pylori
-
gene speE, strain 26695
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography Helicobacter pylori

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine the enzyme possesses a Rossmann-like fold with a distinct active site, the enzyme from Helicobacter pylori lacks the gatekeeping loop that facilitates substrate binding in other PAPTs, active site structure Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosylmethioninamine + putrescine
-
Helicobacter pylori 5'-S-methyl-5'-thioadenosine + spermidine
-
?
2.5.1.16 S-adenosylmethioninamine + putrescine PAPTs are essential for bacterial cell viability Helicobacter pylori 5'-S-methyl-5'-thioadenosine + spermidine
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer dimer interaction in enzyme form A crystals, overview, the enzyme shows an N-terminal beta-stranded domain and a C-terminal Rossmann-like domain, structure comparison Helicobacter pylori
2.5.1.16 tetramer dimer of dimer assembly, enzyme form B crystals, overview, the enzyme shows an N-terminal beta-stranded domain and a C-terminal Rossmann-like domain, structure comparison Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.5.1.16 PAPT
-
Helicobacter pylori
2.5.1.16 putrescine aminopropyltransferase
-
Helicobacter pylori