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Literature summary extracted from

  • Kotaka, M.; Dhaliwal, B.; Ren, J.; Nichols, C.E.; Angell, R.; Lockyer, M.; Hawkins, A.R.; Stammers, D.K.
    Structures of S. aureus thymidylate kinase reveal an atypical active site configuration and an intermediate conformational state upon substrate binding (2006), Protein Sci., 15, 774-784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.9 expression in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.9 For the His-tagged SaTMK crystals, data is collected on beamline ID 14.1 at the ESRF (Grenoble, France). Staphylococcus aureus
2.7.4.9 The data for the untagged SaTMK crystals is collected using Cu Ka radiation from a Rigaku MicroMax 007 generator equipped with aMAR345 image plate. Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.9 thymidine monophosphate + ATP Staphylococcus aureus Thymidylate kinase (TMK) (E.C. 2.7.4.9) is a member of the NMP kinase family and catalyzes the phosphoryl transfer from the preferred phosphoryl donor, ATP, to thymidine monophosphate (TMP), yielding thymidine diphosphate (TDP). The TMK reaction is positioned at the junction of the de novo and salvage pathway of thymidine triphosphate (dTTP) synthesis, with TMK being the last specific enzyme for dTTP synthesis. thymidine diphosphate + ADP
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Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Staphylococcus aureus
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9 Zn2+ charched sepharose column, hydroxyapalite column Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 thymidine monophosphate + ATP Thymidylate kinase (TMK) (E.C. 2.7.4.9) is a member of the NMP kinase family and catalyzes the phosphoryl transfer from the preferred phosphoryl donor, ATP, to thymidine monophosphate (TMP), yielding thymidine diphosphate (TDP). The TMK reaction is positioned at the junction of the de novo and salvage pathway of thymidine triphosphate (dTTP) synthesis, with TMK being the last specific enzyme for dTTP synthesis. Staphylococcus aureus thymidine diphosphate + ADP
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Synonyms

EC Number Synonyms Comment Organism
2.7.4.9 S. aureus thymidylate kinase
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Staphylococcus aureus