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Literature summary extracted from

  • Li, X.; Guo, M.; Fan, J.; Tang, W.; Wang, D.; Ge, H.; Rong, H.; Teng, M.; Niu, L.; Liu, Q.; Hao, Q.
    Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases (2006), Protein Sci., 15, 761-773.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.6 Y3HAO protein is subcloned into the pET-28a expression vector from extracted Saccharomyces cerevisiae genomic DNA, and the Y3HAO protein is highly expressed as a soluble protein in Escherichia coli strain BL21(DE3) with a six-residueHis tag attached to its N-terminus Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.6 hanging drop-vapor diffusion method, 2.4 A resolution Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.6 Fe2+ non-heme ferrous enzyme Saccharomyces cerevisiae
1.13.11.6 Ni2+ two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2 Saccharomyces cerevisiae in the kynurenine pathway 2-amino-3-carboxymuconate semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.6 Saccharomyces cerevisiae
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.6 recombinant Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2 in the kynurenine pathway Saccharomyces cerevisiae 2-amino-3-carboxymuconate semialdehyde
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?

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.6 additional information non-heme enzyme Saccharomyces cerevisiae