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Literature summary extracted from
- Heterologous expression and purification of kynurenine-3-monooxygenase from Pseudomonas fluorescens strain 17400 (2007), Protein Expr. Purif., 51, 324-333.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.13.9 | medicine | the enzyme is an attractive target for the treatment of ischemic stroke | Pseudomonas fluorescens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.9 | functional expression in Escherichia coli strain BL21 (DE3) | Pseudomonas fluorescens |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.9 | disulfide reductants like dithiothreitol or 2-mercaptoethanol stabilize the enzyme | Pseudomonas fluorescens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.9 | 3-nitrobenzoylalanine | - |
Pseudomonas fluorescens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | additional information | - |
additional information | steady-state kinetics and ligand perturbation of flavin fluorescence, overview | Pseudomonas fluorescens | |
| 1.14.13.9 | 0.0085 | - |
NADPH | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 1.14.13.9 | 0.012 | - |
kynurenine | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 1.14.13.9 | 0.034 | - |
O2 | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 1.14.13.9 | 0.053 | - |
kynurenine | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
| 1.14.13.9 | 0.071 | - |
O2 | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
| 1.14.13.9 | 0.091 | - |
NADPH | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 50000 | - |
x * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.9 | kynurenine + NADPH + O2 | Pseudomonas fluorescens | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
| 1.14.13.9 | kynurenine + NADPH + O2 | Pseudomonas fluorescens 17400 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.9 | Pseudomonas fluorescens | - |
- |
- |
| 1.14.13.9 | Pseudomonas fluorescens 17400 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.9 | recombinant enzyme 8.8fold from Escherichia coli strain by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Pseudomonas fluorescens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.9 | microglia | - |
Pseudomonas fluorescens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 3.15 | - |
purified recombinant enzyme | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.9 | kynurenine + NADPH + O2 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | Pseudomonas fluorescens | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
| 1.14.13.9 | kynurenine + NADPH + O2 | enzyme activity depends on the reduction state of the enzyme | Pseudomonas fluorescens | 3-hydroxy-kynurenine + NADP+ + H2O | - |
ir | |
| 1.14.13.9 | kynurenine + NADPH + O2 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | Pseudomonas fluorescens 17400 | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
| 1.14.13.9 | kynurenine + NADPH + O2 | enzyme activity depends on the reduction state of the enzyme | Pseudomonas fluorescens 17400 | 3-hydroxy-kynurenine + NADP+ + H2O | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.9 | ? | x * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.9 | KMO | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 25 | - |
assay at | Pseudomonas fluorescens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | 1.3 | - |
kynurenine | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 1.14.13.9 | 4.9 | - |
kynurenine | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 8.5 | - |
- |
Pseudomonas fluorescens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 7.2 | 9.7 | - |
Pseudomonas fluorescens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 6 | 7 | purified recombinant enzyme, 4°C, stable | Pseudomonas fluorescens |
| 1.14.13.9 | 8 | - |
purified recombinant enzyme, 4°C, 10 h, loss of 21% of activity | Pseudomonas fluorescens |
| 1.14.13.9 | 9 | - |
purified recombinant enzyme, 4°C, 10 h, loss of 32% of activity | Pseudomonas fluorescens |
| 1.14.13.9 | 10 | - |
purified recombinant enzyme, 4°C, 3 h, complete inactivation | Pseudomonas fluorescens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.9 | FAD | flavoprotein | Pseudomonas fluorescens | |
| 1.14.13.9 | NADPH | dependent on | Pseudomonas fluorescens | |
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