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Literature summary extracted from

  • Liu, X.Q.; Yang, X.Q.; Xie, F.H.; Song, L.Y.; Zhang, G.Q.; Qian, S.J.
    On-column refolding and purification of transglutaminase from Streptomyces fradiae expressed as inclusion bodies in Escherichia coli (2007), Protein Expr. Purif., 51, 179-186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 cloned into pET21a and overexpressed in Escherichia coli BL21(DE3). The recombinant TGase is formed as inclusion bodies Streptomyces fradiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 prosuction expression of enzyme as inclusion bodies in Escherichia coli, and purification using an on-column refolding procedure based on cation SP fast flow chromatography. Protein yield is 53%, and 105 mg from 3.2 g wet weight cells, specific activity is 21 U/mg. Refolded protein demonstrates nearly identical abilities compared with native enzyme Streptomyces fradiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces fradiae
-
 expression as inclusion bodies in Escherichia coli
-
2.3.2.13 Streptomyces fradiae Q0GYU0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 recombinant enzyme Streptomyces fradiae
2.3.2.13 recombinant protein expressed as inclusion bodies in Escherichia coli, application of an on-column refolding procedure based on cation SP fast flow chromatography Streptomyces fradiae

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TGase
-
 Streptomyces fradiae