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Literature summary extracted from
- Purification and characterization of a functionally active Mycobacterium tuberculosis prephenate dehydrogenase (2006), Protein Expr. Purif., 49, 151-158.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.3.1.12 | additional information | Mycobacterium tuberculosis PDH is a monofunctional protein, does not possess any chorismate mutase activity unlike many other enteric bacteria | Mycobacterium tuberculosis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.3.1.12 | into tyrA-pET30a and expressed in Escherichia coli BL21(DE3) as His-rMtPDH | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.12 | NaCl | IC50: 0.01 mM | Mycobacterium tuberculosis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.12 | 0.28 | - |
NAD+ | - |
Mycobacterium tuberculosis | |
| 1.3.1.12 | 0.41 | - |
prephenate | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.12 | 31000 | - |
sequence analysis | Mycobacterium tuberculosis |
| 1.3.1.12 | 36420 | - |
MALDI-TOF studies of the recombinant His-rMtPDH | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.12 | Mycobacterium tuberculosis | - |
- |
- |
| 1.3.1.12 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.12 | His-rMtPDH, to near homogeneity by Ni-NTA affinity chromatography, ca. 94% pure | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.12 | prephenate + NAD+ | - |
Mycobacterium tuberculosis | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
| 1.3.1.12 | prephenate + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.1.12 | PDH | - |
Mycobacterium tuberculosis |
| 1.3.1.12 | tyrA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.12 | 37 | - |
- |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.12 | 170.4 | - |
prephenate | - |
Mycobacterium tuberculosis | |
| 1.3.1.12 | 356.1 | - |
NAD+ | - |
Mycobacterium tuberculosis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.12 | 7.9 | - |
- |
Mycobacterium tuberculosis |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.12 | 0.01 | - |
IC50: 0.01 mM | Mycobacterium tuberculosis | NaCl | |
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