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Literature summary extracted from

  • Fan, J.; Wang, D.; Liang, Z.; Guo, M.; Teng, M.; Niu, L.
    Maize uroporphyrinogen III methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification (2006), Protein Expr. Purif., 46, 40-46.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.107 analysis red fluorescent compounds are associated with the recombinant mature SUMT which are identified as sirohydrochlorin and trimethylpyrrocorphin by spectroscopic analysis, slightly alter the protein secondary structure Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.107 Escherichia coli JM109 used for cloning all recombinant plasmids and expressing the pUC18-derived plasmid, Escherichia coli strain S13009 and BL21(DE3) used for expressing the pQE-derived plasmids and the pET-derived plasmid Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.107 additional information deletion mutants, truncated one deleting the C-terminal extra 52 amino acids actively expressed in Escherichia coli, the mature SUMT fusion mutant or the mature SUMT deleting the N-terminal 36 amino acids including glycine-rich region involved directly in SAM binding expressed as an inclusion body in Escherichia coli Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.107 34000
-
 2 * 34000, SDS-PAGE Zea mays
2.1.1.107 52000
-
 gel filtration Zea mays
2.1.1.107 79000
-
 dynamic light scattering assay Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.107 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.107 by Ni–NTA column Zea mays

Subunits

EC Number Subunits Comment Organism
2.1.1.107 homodimer 2 * 34000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.1.1.107 S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase
-
 Zea mays
2.1.1.107 SUMT
-
 Zea mays
2.1.1.107 uroporphyrinogen III methyltransferase
-
 Zea mays