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Literature summary extracted from

  • Madzak, C.; Mimmi, M.C.; Caminade, E.; Brault, A.; Baumberger, S.; Briozzo, P.; Mougin, C.; Jolivalt, C.
    Shifting the optimal pH of activity for a laccase from the fungus Trametes versicolor by structure-based mutagenesis (2006), Protein Eng. Des. Sel., 19, 77-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression of wild-type and mutant enzymes in Yarrowia lipolytica Po1t prototrophic strain Trametes versicolor

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.2 D206A site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme Trametes versicolor
1.10.3.2 D206E site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme Trametes versicolor
1.10.3.2 D206N site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme Trametes versicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.01
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206A Trametes versicolor
1.10.3.2 0.016
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206N Trametes versicolor
1.10.3.2 0.026
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206E Trametes versicolor
1.10.3.2 0.05
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant wild-type enzyme Trametes versicolor
1.10.3.2 0.14
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206A Trametes versicolor
1.10.3.2 0.19
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant wild-type enzyme Trametes versicolor
1.10.3.2 1.63
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206N Trametes versicolor
1.10.3.2 3.28
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206E Trametes versicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ coordination of Cu1 with His458 Trametes versicolor

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes versicolor Q96UT7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes versicolor ?
-
 ?
1.10.3.2 2,5-xylidine + O2 the enzyme also transforms the non-phenolic substrate at pH 5.0, native and recombinant enzymes, wild-type and mutant enzymes, overview Trametes versicolor ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes versicolor 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
1.10.3.2 coniferyl alcohol + O2 the enzyme transforms the non-phenolic substrate at pH 4.5 only when recombinantly expressed in the yeast Yarrowia lipolytica strain Po1t, overview Trametes versicolor ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 30
-
 assay at Trametes versicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.29
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206E Trametes versicolor
1.10.3.2 0.42
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206A Trametes versicolor
1.10.3.2 0.51
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant wild-type enzyme Trametes versicolor
1.10.3.2 0.66
-
 2,6-dimethoxyphenol pH 3.4, 30°C, recombinant mutant D206N Trametes versicolor
1.10.3.2 1.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant wild-type enzyme Trametes versicolor
1.10.3.2 1.6
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206A Trametes versicolor
1.10.3.2 3.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206E Trametes versicolor
1.10.3.2 4.3
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C, recombinant mutant D206N Trametes versicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3 3.4 assay at, dependent on the substrate Trametes versicolor