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Literature summary extracted from

  • Yamada, K.; Gravel, R.A.; Toraya, T.; Matthews, R.G.
    Human methionine synthase reductase is a molecular chaperone for human methionine synthase (2006), Proc. Natl. Acad. Sci. USA, 103, 9476-9481.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.13 expressed in Sf9 insect cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.16.1.8 Homo sapiens
-
 expression by baculovirus-infected insect cells
-
2.1.1.13 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.1.8 after expression by baculovirus-infected insect cells. the flavin mononuleotide cofactor dissociates readily from enzyme upon dilution, hence it is important to keep the concentration high during purification Homo sapiens
2.1.1.13 cobalamin affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 1.94
-
 after 279fold purification at 37°C Homo sapiens
2.1.1.13 2.53
-
 crude cell extract at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.1.8 [methionine synthase]-cob(II)alamin + NADPH + S-adenosylmethionine in presence of methionine synthase reductase, holoenzyme formation from apomethionine synthase and methylcobalamin is significantly enhanced due to stabilization of apomethionine synthase. In addition to reductase activity, methionine synthase reductase serves as a special chaperone for methionine synthase. It also has reductase activity for the reaction of aquacobalamin to cob(II)alamin Homo sapiens [methionine synthase]-methylcob(I)alamin + NADPH + S-adenosylhomocysteine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Homo sapiens tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.16.1.8 More in addition to reductase activity, methionine synthase reductase serves as a special chaperone for methionine synthase Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.13 MS
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.13 37
-
 the apoenzyme preincubated in the absence of methyl synthase reductase almost completely loses the ability to form holoenzyme after the subsequent addition of methylcobalamin and incubation at 37°C for 10 min Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 vitamin B12 required for activity Homo sapiens