Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, S.; Chang, J.S.; Herberg, J.T.; Horng, M.M.; Tomich, P.K.; Lin, A.H.; Marotti, K.R.
    Allosteric inhibition of Staphylococcus aureus D-alanine:D-alanine ligase revealed by crystallographic studies (2006), Proc. Natl. Acad. Sci. USA, 103, 15178-15183.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.4 crystal structure of Staphylococcus aureus D-alanine:D-alanine ligase and its cocrystal structures with 3-chloro-2,2-dimethyl-N-4(trifluoromethyl)phenylpropanamide and with ADP at resolutions of 2.0 A, 2.2 A, and 2.6 A, respectively Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.4 3-chloro-2,2-dimethyl-N-[4(trifluoromethyl)phenyl]propanamide the inhibitor binds to a hydrophobic pocket at the interface of the first and the third domain. This inhibitor-binding pocket is adjacent to the first D-alanine substrate site but does not overlap with any substrate sites Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.4 0.06
-
 ATP
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.4 ATP + D-alanine Staphylococcus aureus D-alanine:D-alanine ligase is an essential enzyme in bacterial cell wall biosynthesis and an important target for developing new antibiotics ADP + phosphate + D-alanyl-D-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.4 Staphylococcus aureus Q5HEB7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.4 ATP + D-alanine
-
 Staphylococcus aureus ADP + phosphate + D-alanyl-D-alanine
-
 ?
6.3.2.4 ATP + D-alanine D-alanine:D-alanine ligase is an essential enzyme in bacterial cell wall biosynthesis and an important target for developing new antibiotics Staphylococcus aureus ADP + phosphate + D-alanyl-D-alanine
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.2.4 0.04
-
 3-chloro-2,2-dimethyl-N-[4(trifluoromethyl)phenyl]propanamide
-
 Staphylococcus aureus