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Literature summary extracted from

  • Thomas, C.J.; Du, X.; Li, P.; Wang, Y.; Ross, E.M.; Sprang, S.R.
    Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit (2004), Proc. Natl. Acad. Sci. USA, 101, 7560-7565.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.1 RGS4
-
 Mammalia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.1 into vector for expression in Escherichia coli Mammalia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.1 crystal structure of mutant K180P-Galphai1 bound to a GTP analog Mammalia

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.1 G202A mutation of Galphai1 accelerates the rates of GTP hydrolysis and conformational change Mammalia
3.6.5.1 G202A, K180P mutations of Galphai1 accelerate the rates of GTP hydrolysis Mammalia
3.6.5.1 K180A mutant of Galphai1, rate of GTP hydrolysis similar to wild-type Mammalia
3.6.5.1 K180P mutation of Galphai1 increases the rate of conformational change and decreases the rates of GTP hydrolysis Mammalia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.5.1 Mg2+
-
 Mammalia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.1 GTP + H2O Mammalia
-
 GDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.1 Mammalia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.1 using a Ni-NTA column Mammalia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.1 GTP + H2O
-
 Mammalia GDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.5.1 heterotrimer Galpha subunit, GDP-bound inactive, GTP-bound active and betagamma dimer Mammalia

Synonyms

EC Number Synonyms Comment Organism
3.6.5.1 GTP phosphohydrolase
-
 Mammalia
3.6.5.1 heterotrimeric G-protein GTPase
-
 Mammalia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.5.1 8
-
 single-turnover GTPase assay Mammalia