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Literature summary extracted from

  • Bahmed, K.; Quiles, F.; Wathier, M.; Bonaly, R.; Benallaoua, S.; Pucci, B.; Coulon, J.
    Use of dansyl N-acetyl glucosamine as substrate for chitin synthetase activities (2005), Proc. Biochem., 40, 2523-2529.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.16 Nikkomycin 0.015 for chitin synthetase III; IC50: 0.022 mM for chitin synthetase II; IC50: 0.032 mM for chitin synthetase I Kluyveromyces bulgaricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 0.0133
-
 6-O-dansyl-N-acetylglucosamine chitin synthetase I Kluyveromyces bulgaricus
2.4.1.16 0.2
-
 6-O-dansyl-N-acetylglucosamine chitin synthetase III Kluyveromyces bulgaricus
2.4.1.16 0.5
-
 6-O-dansyl N-acetylglucosamine chitin synthetase II Kluyveromyces bulgaricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Kluyveromyces bulgaricus
-
 ATCC 96631
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.16 0.0025
-
 chitin synthetase III, activity at the end of exponential growth phase Kluyveromyces bulgaricus
2.4.1.16 0.0028
-
 chitin synthetase II, activity at the end of exponential growth phase Kluyveromyces bulgaricus
2.4.1.16 0.008
-
 chitin synthetase I, activity at the end of exponential growth phase Kluyveromyces bulgaricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 6-O-dansyl-N-acetylglucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Kluyveromyces bulgaricus ?
-
 ?
2.4.1.16 acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Kluyveromyces bulgaricus UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.16 chitin synthetase I
-
 Kluyveromyces bulgaricus
2.4.1.16 chitin synthetase II
-
 Kluyveromyces bulgaricus
2.4.1.16 chitin synthetase III
-
 Kluyveromyces bulgaricus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.16 0.022
-
 IC50: 0.022 mM for chitin synthetase II Kluyveromyces bulgaricus Nikkomycin
2.4.1.16 0.032
-
 IC50: 0.032 mM for chitin synthetase I Kluyveromyces bulgaricus Nikkomycin