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Literature summary extracted from

  • Ziegler, J.; Diaz-Chavez, M.L.; Kramell, R.; Ammer, C.; Kutchan, T.M.
    Comparative macroarray analysis of morphine containing Papaver somniferum and eight morphine free Papaver species identifies an O-methyltransferase involved in benzylisoquinoline biosynthesis (2005), Planta, 222, 458-471.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.116 additional information the fragment A11A1 with increased expression in Papaver somniferum plants compared to other Papaver species shows the highest homology (75% identity on protein level) to the 4'-OMT from Coptis japonica Papaver somniferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.116 into vector pQE31 containing six histidines as an N-terminal tag and overexpressed in Escherichia coli SG13009 Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.116 0.031
-
(R,S)-6-O-methylnorlaudanosoline
-
Papaver somniferum
2.1.1.116 0.037
-
(R,S)-norlaudanosoline
-
Papaver somniferum
2.1.1.116 0.039
-
(R,S)-laudanosoline
-
Papaver somniferum
2.1.1.116 0.081
-
(R,S)-3'-hydroxy-N-methylcoclaurine
-
Papaver somniferum
2.1.1.116 0.136
-
S-adenosyl-L-methionine with (R,S)-norlaudanosoline as substrate Papaver somniferum
2.1.1.116 0.179
-
S-adenosyl-L-methionine with (R,S)-6-O-methylnorlaudanosoline as substrate Papaver somniferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.116 39800
-
2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis Papaver somniferum
2.1.1.116 42500
-
2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis Papaver somniferum
2.1.1.116 75000
-
gel filtration Papaver somniferum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.116 Papaver somniferum
-
varieties Paso Munich, Noscapine and NOPA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.116 on cobalt affinity column Papaver somniferum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.116 seedling
-
Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.116 additional information only tetrahydroxylated tetrahydrobenzylisoquinolines are accepted as substrates, methylation is tolerated only at the 6-hydroxy position, no methylation when catechol and guaiacol or the phenylpropanoids caffeic and ferulic acid are used as substrate Papaver somniferum ?
-
?
2.1.1.116 S-adenosyl-L-methionine + (R,S)-3'-hydroxy-N-methylcoclaurine
-
Papaver somniferum ?
-
?
2.1.1.116 S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline
-
Papaver somniferum ?
-
?
2.1.1.116 S-adenosyl-L-methionine + (R,S)-laudanosoline
-
Papaver somniferum ?
-
?
2.1.1.116 S-adenosyl-L-methionine + (R,S)-norlaudanosoline
-
Papaver somniferum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.116 homodimer 2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis Papaver somniferum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.116 4'-OMT
-
Papaver somniferum
2.1.1.116 S-adenosyl-L-methionine:(R,S)-3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase
-
Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.116 40
-
with (R,S)-3'-hydroxy-N-methylcoclaurine as substrate Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.116 7
-
with (R,S)-3'-hydroxy-N-methylcoclaurine as substrate Papaver somniferum