EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.116 | additional information | the fragment A11A1 with increased expression in Papaver somniferum plants compared to other Papaver species shows the highest homology (75% identity on protein level) to the 4'-OMT from Coptis japonica | Papaver somniferum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.116 | into vector pQE31 containing six histidines as an N-terminal tag and overexpressed in Escherichia coli SG13009 | Papaver somniferum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.116 | 0.031 | - |
(R,S)-6-O-methylnorlaudanosoline | - |
Papaver somniferum | |
2.1.1.116 | 0.037 | - |
(R,S)-norlaudanosoline | - |
Papaver somniferum | |
2.1.1.116 | 0.039 | - |
(R,S)-laudanosoline | - |
Papaver somniferum | |
2.1.1.116 | 0.081 | - |
(R,S)-3'-hydroxy-N-methylcoclaurine | - |
Papaver somniferum | |
2.1.1.116 | 0.136 | - |
S-adenosyl-L-methionine | with (R,S)-norlaudanosoline as substrate | Papaver somniferum | |
2.1.1.116 | 0.179 | - |
S-adenosyl-L-methionine | with (R,S)-6-O-methylnorlaudanosoline as substrate | Papaver somniferum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.116 | 39800 | - |
2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis | Papaver somniferum |
2.1.1.116 | 42500 | - |
2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis | Papaver somniferum |
2.1.1.116 | 75000 | - |
gel filtration | Papaver somniferum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.116 | Papaver somniferum | - |
varieties Paso Munich, Noscapine and NOPA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.116 | on cobalt affinity column | Papaver somniferum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.116 | seedling | - |
Papaver somniferum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.116 | additional information | only tetrahydroxylated tetrahydrobenzylisoquinolines are accepted as substrates, methylation is tolerated only at the 6-hydroxy position, no methylation when catechol and guaiacol or the phenylpropanoids caffeic and ferulic acid are used as substrate | Papaver somniferum | ? | - |
? | |
2.1.1.116 | S-adenosyl-L-methionine + (R,S)-3'-hydroxy-N-methylcoclaurine | - |
Papaver somniferum | ? | - |
? | |
2.1.1.116 | S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline | - |
Papaver somniferum | ? | - |
? | |
2.1.1.116 | S-adenosyl-L-methionine + (R,S)-laudanosoline | - |
Papaver somniferum | ? | - |
? | |
2.1.1.116 | S-adenosyl-L-methionine + (R,S)-norlaudanosoline | - |
Papaver somniferum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.116 | homodimer | 2 * 42500, SDS-PAGE, 2 * 39800, sequence analysis | Papaver somniferum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.116 | 4'-OMT | - |
Papaver somniferum |
2.1.1.116 | S-adenosyl-L-methionine:(R,S)-3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase | - |
Papaver somniferum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.116 | 40 | - |
with (R,S)-3'-hydroxy-N-methylcoclaurine as substrate | Papaver somniferum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.116 | 7 | - |
with (R,S)-3'-hydroxy-N-methylcoclaurine as substrate | Papaver somniferum |