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Literature summary extracted from

  • Cervantes-Cervantes, M.; Gallagher, C.E.; Zhu, C.; Wurtzel, E.T.
    Maize cDNAs expressed in endosperm encode functional farnesyl diphosphate synthase with geranylgeranyl diphosphate synthase activity (2006), Plant Physiol., 141, 220-231.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.29 a short and a long clone, which differ in the substrate specificity, DNA and amino acid sequence determination and analysis, genetic organization, functional complementation of an Escherichia coli strain carrying an Erwinia uredovora gene cluster encoding all pathway enzymes needed for carotenoid biosynthesis, except for GGPPS, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+
-
 Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 trans,trans-farnesyl diphosphate + isopentenyl diphosphate Zea mays
-
 diphosphate + geranylgeranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Zea mays Q548K3 cv. B73
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.29 endosperm
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 additional information the bifunctional enzyme is encoded by two clones of different lengths, exhibiting different preferences in farnesyl diphosphate synthesis and geranylgeranyl diphosphate synthesis, overview Zea mays ?
-
 ?
2.5.1.29 trans,trans-farnesyl diphosphate + isopentenyl diphosphate
-
 Zea mays diphosphate + geranylgeranyl diphosphate
-
 ?
2.5.1.29 trans,trans-farnesyl diphosphate + isopentenyl diphosphate the N-terminal sequence affects the ratio of FPP to GGPP Zea mays diphosphate + geranylgeranyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.29 geranylgeranyl pyrophosphate synthase
-
 Zea mays
2.5.1.29 GGPPS
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.29 37
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 7.4
-
 assay at Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.29 6.5 10.5 activity range Zea mays