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Literature summary extracted from

  • Kang, S.; Kang, K.; Chung, G.C.; Choi, D.; Ishihara, A.; Lee, D.; Back, K.
    Functional analysis of the amine substrate specificity domain of pepper tyramine and serotonin N-hydroxycinnamoyltransferases (2006), Plant Physiol., 140, 704-715.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.110 expression in Escherichia coli Capsicum annuum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.110 F145Y KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 3fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin Capsicum annuum
2.3.1.110 F145Y/Y147F KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 4.2fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin Capsicum annuum
2.3.1.110 additional information a series of chimeric genes is constructed by reciprocal exchange of DNA segments between the serotonin N-hydroxycinnamoyltransferase and tyramine N-hydroxycinnamoyltransferase cDNAs. Functional characterization of the recombinant chimeric proteins revealed that the amino acid residues 129 to 165 of serotonin N-hydroxycinnamoyltransferase and the corresponding residues 125 to 160 in tyramine N-hydroxycinnamoyltransferase are critical structural determinants for amine substrate specificity Capsicum annuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.110 0.02
-
 4-coumaroyl-CoA 30°C, pH 8.5, recombinant enzyme Capsicum annuum
2.3.1.110 0.04
-
 tyramine 30°C, pH 8.5, recombinant enzyme Capsicum annuum
2.3.1.110 0.053
-
 feruloyl-CoA 30°C, pH 8.5, recombinant enzyme Capsicum annuum
2.3.1.110 0.17
-
 tyramine 30°C, pH 8.5, mutant enzyme F145Y Capsicum annuum
2.3.1.110 0.17
-
 tyramine 30°C, pH 8.5, mutant enzyme F145Y/Y147F Capsicum annuum
2.3.1.110 0.779
-
 dopamine 30°C, pH 8.5, recombinant enzyme Capsicum annuum
2.3.1.110 20.98
-
 serotonin 30°C, pH 8.5, mutant enzyme F145Y Capsicum annuum
2.3.1.110 24
-
 tryptamine 30°C, pH 8.5, recombinant enzyme Capsicum annuum
2.3.1.110 25.44
-
 serotonin 30°C, pH 8.5, mutant enzyme F145Y/Y147F Capsicum annuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.110 28221
-
 x * 28221, calculatred from nucleotide sequence Capsicum annuum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.110 Capsicum annuum Q5D8C0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.110 additional information THT mRNA is expressed constitutively in all pepper organs Capsicum annuum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.110 4-coumaroyl-CoA + tyramine
-
 Capsicum annuum CoA + N-(4-coumaroyl)tyramine
-
 ?
2.3.1.110 caffeoyl-CoA + serotonin
-
 Capsicum annuum CoA + N-caffeoylserotonin
-
 ?
2.3.1.110 feruloyl-CoA + dopamine
-
 Capsicum annuum CoA + feruloyldopamine
-
 ?
2.3.1.110 feruloyl-CoA + tryptamine
-
 Capsicum annuum CoA + feruloyltryptamine
-
 ?
2.3.1.110 feruloyl-CoA + tyramine
-
 Capsicum annuum CoA + feruloyltyramine
-
 ?
2.3.1.110 additional information wild-type enzyme shows no activity with serotonin, mutant enzyms F145Y and F145Y/Y147F show activity with serotonin Capsicum annuum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.110 ? x * 28221, calculatred from nucleotide sequence Capsicum annuum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.110 THT
-
 Capsicum annuum
2.3.1.110 tyramine N-hydroxycinnamoyltransferase
-
 Capsicum annuum