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Literature summary extracted from
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms (2005), Plant Physiol., 139, 1935-1945.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.14.86 | biotechnology | P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase | Oryza sativa Japonica Group |
| 1.14.14.86 | biotechnology | P2 may provide a tool to investigate the regulation of GA metabolism for plant growth and development via affection of ent-kaurene oxidase | Oryza sativa |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.86 | DNA and amino acid sequence determination and analysis, sequence comparisons | Oryza sativa Japonica Group |
| 1.14.14.86 | KOS2, DNA and amino acid sequence determination and analysis, sequence comparisons | Oryza sativa Japonica Group |
| 1.14.14.86 | KOS3, DNA and amino acid sequence determination and analysis, sequence comparisons | Oryza sativa |
| 1.14.14.86 | KOS4, DNA and amino acid sequence determination and analysis, sequence comparisons | Oryza sativa Japonica Group |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.86 | (-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Oryza sativa Japonica Group | the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins | (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |  |
| 1.14.14.86 | (-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Oryza sativa | the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins | (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.86 | additional information | Oryza sativa Japonica Group | the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview | ? | - |
? | |
| 1.14.14.86 | additional information | Oryza sativa | the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.86 | Oryza sativa | Q68YV8 | isozyme KOS3; ssp. japonica, isozymes KOS1-KOS4 | - |
| 1.14.14.86 | Oryza sativa Japonica Group | Q6GZ42 | isozyme KOS1; isozymes KOS1-KOS4 | - |
| 1.14.14.86 | Oryza sativa Japonica Group | Q68YV9 | isozyme KOS2; isozymes KOS1-KOS4 | - |
| 1.14.14.86 | Oryza sativa Japonica Group | Q68YV7 | isozyme KOS4; isozymes KOS1-KOS4 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.86 | leaf | - |
Oryza sativa Japonica Group | - |
| 1.14.14.86 | leaf | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.86 | additional information | - |
gibberellic acid content in healthy and rice dwarf virus-infected plants | Oryza sativa Japonica Group |
| 1.14.14.86 | additional information | - |
gibberellic acid content in healthy and rice dwarf virus-infected plants | Oryza sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.86 | (-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins | Oryza sativa Japonica Group | (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.86 | (-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | the enzyme plays a key role in the biosynthesis of plant growth hormones gibberellins | Oryza sativa | (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.86 | (-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | a three step oxidation | Oryza sativa Japonica Group | (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.86 | (-)-kaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | a three step oxidation | Oryza sativa | (-)-kaurenoic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
| 1.14.14.86 | additional information | the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview | Oryza sativa Japonica Group | ? | - |
? | |
| 1.14.14.86 | additional information | the rice dwarf virus P2 protein interacts with ent-kaurene oxidase isozymes in vivo, leading to reduced enzyme expression and biosynthesis of gibberellins and rice dwarf symptoms, mechanism, overview | Oryza sativa | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.86 | ent-kaurene oxidase-like protein | - |
Oryza sativa Japonica Group |
| 1.14.14.86 | ent-kaurene oxidase-like protein | - |
Oryza sativa |
| 1.14.14.86 | KOS1 | - |
Oryza sativa Japonica Group |
| 1.14.14.86 | KOS2 | - |
Oryza sativa Japonica Group |
| 1.14.14.86 | KOS3 | - |
Oryza sativa |
| 1.14.14.86 | KOS4 | - |
Oryza sativa Japonica Group |
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