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Literature summary extracted from

  • Zhao, P.; Inoue, K.; Kouno, I.; Yamamoto, H.
    Characterization of leachianone G 2''-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures (2003), Plant Physiol., 133, 1306-1313.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.71 0.0023
-
 leachianone G pH 8.8, 30°C Sophora flavescens
2.5.1.71 0.059
-
 dimethylallyl diphosphate pH 8.8, 30°C Sophora flavescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.142 endoplasmic reticulum
-
 Sophora flavescens 5783
-
1.14.14.142 additional information subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview Sophora flavescens
-
-
2.5.1.70 chloroplast
-
 Sophora flavescens 9507
-
2.5.1.70 microsome
-
 Sophora flavescens
-
-
2.5.1.70 additional information subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview Sophora flavescens
-
-
2.5.1.71 chloroplast
-
 Sophora flavescens 9507
-
2.5.1.71 membrane bound, determination of enzyme distibution in membranes Sophora flavescens 16020
-
2.5.1.71 additional information subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview Sophora flavescens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.70 Mg2+
-
 Sophora flavescens
2.5.1.71 Ca2+ 25% of the activity with Mg2+ Sophora flavescens
2.5.1.71 Co2+ 5% of the activity with Mg2+ Sophora flavescens
2.5.1.71 Cu2+ 4% of the activity with Mg2+ Sophora flavescens
2.5.1.71 Fe2+ 5% of the activity with Mg2+ Sophora flavescens
2.5.1.71 Mg2+ required, optimal at 10 mM, other divalent cations can only partially substitute for Mg2+, overview Sophora flavescens
2.5.1.71 Mn2+ 15% of the activity with Mg2+ Sophora flavescens
2.5.1.71 Ni2+ 5% of the activity with Mg2+ Sophora flavescens
2.5.1.71 Zn2+ 10% of the activity with Mg2+ Sophora flavescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.142 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 Sophora flavescens the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin Sophora flavescens
-
 diphosphate + sophoraflavanone B
-
 ?
2.5.1.71 dimethylallyl diphosphate + leachianone G Sophora flavescens the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant diphosphate + sophoraflavanone G
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.142 Sophora flavescens
-
-
-
2.5.1.70 Sophora flavescens
-
-
-
2.5.1.71 Sophora flavescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.142 native enzyme partially by microsome preparation Sophora flavescens
2.5.1.71 native enzyme partially by microsome preparation Sophora flavescens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.70 dimethylallyl diphosphate + (-)-(2S)-naringenin = diphosphate + sophoraflavanone B reaction mechanism Sophora flavescens
a
2.5.1.71 dimethylallyl diphosphate + leachianone G = diphosphate + sophoraflavanone G reaction mechanism Sophora flavescens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.142 cell suspension culture
-
 Sophora flavescens
-
2.5.1.70 cell suspension culture
-
 Sophora flavescens
-
2.5.1.71 cell suspension culture
-
 Sophora flavescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.70 0.001
-
 microsomes Sophora flavescens
2.5.1.71 0.00014
-
 crude cell extract Sophora flavescens
2.5.1.71 0.06
-
 microsomes Sophora flavescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.142 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Sophora flavescens leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.142 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview Sophora flavescens leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin
-
 Sophora flavescens diphosphate + sophoraflavanone B
-
 ?
2.5.1.70 3,3-dimethylallyl diphosphate + (2S)-naringenin i.e. (S)-4',5,7-trihydroxy-flavanone Sophora flavescens diphosphate + sophoraflavanone B
-
 ?
2.5.1.71 dimethylallyl diphosphate + euchrenone a7 54% of the activity with leachianone G Sophora flavescens diphosphate + ?
-
 ?
2.5.1.71 dimethylallyl diphosphate + leachianone G the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant Sophora flavescens diphosphate + sophoraflavanone G
-
 ?
2.5.1.71 dimethylallyl diphosphate + leachianone G the enzyme is a prenyl side-chain elongation enzyme, the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone, the enzyme is absolutely specific for dimethylallyl diphosphate as prenyl donor and highly specific for leachianone G, the 2'-hydroxy function in leachianone G is indispensable to the activity Sophora flavescens diphosphate + sophoraflavanone G
-
 ?
2.5.1.71 additional information no activity with isopentenyl diphosphate and geranyl diphosphate as prenyl donors, and with kenusanone I, 8-dimethylallylnaringenin, and isovabachin as acceptor substrates, overview Sophora flavescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.142 8-dimethylallylnaringenin 2'-hydroxylase
-
 Sophora flavescens
2.5.1.71 leachianone G 2''-dimethylallyltransferase
-
 Sophora flavescens
2.5.1.71 LG 2''-dimethylallyltransferase
-
 Sophora flavescens
2.5.1.71 LGDT
-
 Sophora flavescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.71 30
-
 assay at Sophora flavescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.71 8.8
-
-
 Sophora flavescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.71 7.9 10 50% of maximal activity within this range, almost inactive at pH 6.8, pH profile, overview Sophora flavescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.142 additional information the enzyme is a cytochrome P450 monooxygenase Sophora flavescens