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Literature summary extracted from

  • Teuber, M.; Azemi, M.E.; Namjoyan, F.; Meier, A.C.; Wodak, A.; Brandt, W.; Draeger, B.
    Putrescine N-methyltransferases-a structure-function analysis (2007), Plant Mol. Biol., 63, 787-801.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.53 additional information protein model of PMT based on the crystal structure of SPDS suggests that overall protein folds are comparable, the respective cosubstrates S-adenosylmethionine and decarboxylated S-adenosylmethionine, however, appear to bind differentially to the active sites of both enzymes, and the substrate putrescine adopts a different position Datura stramonium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.53 expressed in Rosetta-gami (DE3) cells Solanum lycopersicum
2.1.1.53 expressed in Rosetta-gami (DE3) cells Calystegia sepium
2.1.1.53 expressed in Rosetta-gami (DE3) cells Physalis divaricata
2.1.1.53 expressed in Rosetta-gami (DE3) cells Solanum dulcamara
2.1.1.53 expressed in Rosetta-gami (DE3) cells Datura inoxia
2.1.1.53 expressed in Rosetta-gami (DE3) cells Datura stramonium
2.1.1.53 into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells Physalis divaricata
2.1.1.53 into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells Calystegia sepium
2.1.1.53 into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells Solanum dulcamara
2.1.1.53 into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells Datura inoxia
2.1.1.53 into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells Datura stramonium
2.1.1.53 into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.53 I136V reduced PMT activity, about half of the wild-type activity Datura stramonium
2.1.1.53 I141D no PMT activity Datura stramonium
2.1.1.53 I141D mutant shows no activity Datura stramonium
2.1.1.53 T117Q no PMT activity Datura stramonium
2.1.1.53 T117Q mutant shows no activity Datura stramonium
2.1.1.53 T117Q/I141D no PMT activity Datura stramonium
2.1.1.53 T117Q/I141D mutant shows no activity Datura stramonium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.53 0.113
-
 putrescine
-
 Solanum lycopersicum
2.1.1.53 0.12
-
 putrescine PMT1 Calystegia sepium
2.1.1.53 0.12
-
 putrescine isoenzyme PMT1 Calystegia sepium
2.1.1.53 0.169
-
 putrescine
-
 Solanum dulcamara
2.1.1.53 0.281
-
 putrescine
-
 Datura stramonium

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.53 Calystegia sepium
-
-
-
2.1.1.53 Calystegia sepium Q2KTH3
-
-
2.1.1.53 Datura inoxia Q2KTH1
-
-
2.1.1.53 Datura inoxia Q2KTH2
-
-
2.1.1.53 Datura stramonium Q70EW6
-
-
2.1.1.53 Physalis divaricata Q2KTH0
-
-
2.1.1.53 Solanum dulcamara
-
-
-
2.1.1.53 Solanum lycopersicum
-
-
-
2.1.1.53 Solanum lycopersicum Q2KTH4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.53 gel filtration, approximately 95% pure Physalis divaricata
2.1.1.53 gel filtration, approximately 95% pure Calystegia sepium
2.1.1.53 gel filtration, approximately 95% pure Solanum dulcamara
2.1.1.53 gel filtration, approximately 95% pure Datura inoxia
2.1.1.53 gel filtration, approximately 95% pure Datura stramonium
2.1.1.53 gel filtration, approximately 95% pure Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.53 root
-
 Solanum lycopersicum
-
2.1.1.53 root
-
 Calystegia sepium
-
2.1.1.53 root
-
 Physalis divaricata
-
2.1.1.53 root
-
 Solanum dulcamara
-
2.1.1.53 root
-
 Datura inoxia
-
2.1.1.53 root
-
 Datura stramonium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Solanum lycopersicum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Calystegia sepium S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Physalis divaricata S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Solanum dulcamara S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Datura inoxia S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Datura stramonium S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.53 PMT
-
 Solanum lycopersicum
2.1.1.53 PMT
-
 Physalis divaricata
2.1.1.53 PMT
-
 Solanum dulcamara
2.1.1.53 PMT
-
 Datura stramonium
2.1.1.53 PMT1
-
 Calystegia sepium
2.1.1.53 PMT1
-
 Datura inoxia
2.1.1.53 PMT2
-
 Calystegia sepium
2.1.1.53 PMT2
-
 Datura inoxia
2.1.1.53 putrescine N-methyltransferase
-
 Solanum lycopersicum
2.1.1.53 putrescine N-methyltransferase
-
 Calystegia sepium
2.1.1.53 putrescine N-methyltransferase
-
 Physalis divaricata
2.1.1.53 putrescine N-methyltransferase
-
 Solanum dulcamara
2.1.1.53 putrescine N-methyltransferase
-
 Datura inoxia
2.1.1.53 putrescine N-methyltransferase
-
 Datura stramonium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.53 30
-
 assay at Solanum lycopersicum
2.1.1.53 30
-
 assay at Calystegia sepium
2.1.1.53 30
-
 assay at Physalis divaricata
2.1.1.53 30
-
 assay at Solanum dulcamara
2.1.1.53 30
-
 assay at Datura inoxia
2.1.1.53 30
-
 assay at Datura stramonium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.53 0.047
-
 putrescine
-
 Solanum dulcamara
2.1.1.53 0.086
-
 putrescine PMT2 Calystegia sepium
2.1.1.53 0.086
-
 putrescine isoenzyme PMT2 Calystegia sepium
2.1.1.53 0.16
-
 putrescine PMT1 Calystegia sepium
2.1.1.53 0.16
-
 putrescine isoenzyme PMT1 Calystegia sepium
2.1.1.53 0.19
-
 putrescine
-
 Solanum lycopersicum
2.1.1.53 0.198
-
 putrescine
-
 Physalis divaricata
2.1.1.53 0.359
-
 putrescine
-
 Datura inoxia
2.1.1.53 0.383
-
 putrescine
-
 Datura inoxia
2.1.1.53 0.383
-
 putrescine PMT1 Datura inoxia
2.1.1.53 0.388
-
 putrescine
-
 Datura stramonium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.53 9
-
-
 Physalis divaricata
2.1.1.53 9
-
-
 Calystegia sepium
2.1.1.53 9
-
-
 Solanum dulcamara
2.1.1.53 9
-
-
 Datura inoxia
2.1.1.53 9
-
-
 Datura stramonium
2.1.1.53 9
-
-
 Solanum lycopersicum
2.1.1.53 9
-
 assay at Solanum lycopersicum
2.1.1.53 9
-
 assay at Calystegia sepium
2.1.1.53 9
-
 assay at Physalis divaricata
2.1.1.53 9
-
 assay at Solanum dulcamara
2.1.1.53 9
-
 assay at Datura inoxia
2.1.1.53 9
-
 assay at Datura stramonium