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Literature summary extracted from

  • Deavours, B.E.; Liu, C.J.; Naoumkina, M.A.; Tang, Y.; Farag, M.A.; Sumner, L.W.; Noel, J.P.; Dixon, R.A.
    Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula (2006), Plant Mol. Biol., 62, 715-733.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.212 expression in Escherichia coli Medicago truncatula

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.150 Medicago truncatula
-
-
-
2.1.1.150 Medicago truncatula Q06YR3
-
-
2.1.1.150 Medicago truncatula Q06YR4
-
-
2.1.1.212 Medicago truncatula Q29U70
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.212
-
 Medicago truncatula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.150 additional information no activity with 6,7-dihydroxy-4'-methoxyisoflavone Medicago truncatula ?
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein. 49% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein) Medicago truncatula S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein. 61% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago truncatula S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone preferred substrate Medicago truncatula S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone 35% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago truncatula S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone 44% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein) Medicago truncatula S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,3',4'-trihydroxyisoflavone 41% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein) Medicago truncatula S-adenosyl-L-homocysteine + 3',4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,3',4'-trihydroxyisoflavone 43% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago truncatula S-adenosyl-L-homocysteine + 3',4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone i.e. glycitein, 39% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone i.e. glycitein, 52% the activity with 6,7,4'-trihydroxyisoflavone (i.e. daidzein) Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxy-6-methoxyisoflavone i.e. glycitein, preferred substrate Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-6,7-dimethoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavanone i.e. dihydrodaidzein. 15.6% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein) Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavanone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavanone i.e. dihydrodaidzein. 70% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein) Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavanone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. 21% of the activity with 7,4'-dihydroxy-6-methoxyisoflavone (i.e. glycitein) Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. formononetin ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. Preferred substrate Medicago truncatula S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone
-
 ?
2.1.1.212 additional information low activity with dihydrodaidzein. 4'-Methoxy, 7-methoxy, and 4',7-dimethoxy products are formed in the reaction with dihydrodaidzein. No activity with: 6,7,4'-trihydroxyisoflavone, 6,7'-dihydroxy,4'-methoxyisoflavone, 7,3',4'-trihydroxyisoflavone, daidzein, genistein, glycitein, coumestrol, apigenin, 7,4'-dihydroxyflavone and myricetin Medicago truncatula ?
-
 ?
2.1.1.212 S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
-
 Medicago truncatula S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.150 MtIOMT1
-
 Medicago truncatula
2.1.1.150 MtIOMT2
-
 Medicago truncatula
2.1.1.150 MtIOMT3
-
 Medicago truncatula
2.1.1.212 MtIOMT5
-
 Medicago truncatula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.212 30
-
 assay at Medicago truncatula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.212 7.4
-
 assay at Medicago truncatula