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Literature summary extracted from

  • Dauvillee, D.; Chochois, V.; Steup, M.; Haebel, S.; Eckermann, N.; Ritte, G.; Ral, J.P.; Colleoni, C.; Hicks, G.; Wattebled, F.; Deschamps, P.; dHulst, C.; Lienard, L.; Cournac, L.; Putaux, J.L.; Dupeyre, D.; Ball, S.G.
    Plastidial phosphorylase is required for normal starch synthesis in Chlamydomonas reinhardtii (2006), Plant J., 48, 274-285.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.1 additional information enzyme mutants display a significant decrease in amounts of starch during storage that correlates with the accumulation of abnormally shaped granules containing a modified amylopekti structure and a high amylose content Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADP-glucose maximal inhibition achieved is 70%; maximal inhibition achieved is 95% Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.41
-
 alpha-D-glucose 1-phosphate pH 7.0, 30°C Chlamydomonas reinhardtii
2.4.1.1 0.68
-
 alpha-D-glucose 1-phosphate pH 7.0, 30°C Chlamydomonas reinhardtii
2.4.1.1 1.6
-
 phosphate pH 7.0, 30°C Chlamydomonas reinhardtii
2.4.1.1 1.9
-
 phosphate pH 7.0, 30°C Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 plastid
-
 Chlamydomonas reinhardtii 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 91000
-
 2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated Chlamydomonas reinhardtii
2.4.1.1 94000
-
 2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated Chlamydomonas reinhardtii
2.4.1.1 106000
-
 2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme Chlamydomonas reinhardtii
2.4.1.1 108000
-
 2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme Chlamydomonas reinhardtii
2.4.1.1 182000
-
 gel filtration Chlamydomonas reinhardtii
2.4.1.1 214000
-
 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 additional information Chlamydomonas reinhardtii enzyme is involved in storage starch synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Chlamydomonas reinhardtii Q2VA40 isoform PhoA
-
2.4.1.1 Chlamydomonas reinhardtii Q2VA41 isoform PhoB, encoded by STA4
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.1 proteolytic modification
-
 Chlamydomonas reinhardtii
2.4.1.1 proteolytic modification cleavage of a 55 amino acid transit peptide Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (maltooligosaccaride)n-1 + alpha-D-glucose 1-phosphate
-
 Chlamydomonas reinhardtii (maltooligosaccharide)n + phosphate
-
 r
2.4.1.1 (maltooligosaccaride)n-1 + alpha-D-glucose 1-phosphate preferred substrate Chlamydomonas reinhardtii (maltooligosaccharide)n + phosphate
-
 r
2.4.1.1 glycogen + alpha-D-glucose 1-phosphate
-
 Chlamydomonas reinhardtii glycogen + phosphate
-
 r
2.4.1.1 additional information enzyme is involved in storage starch synthesis Chlamydomonas reinhardtii ?
-
 ?
2.4.1.1 starch + alpha-D-glucose 1-phosphate
-
 Chlamydomonas reinhardtii starch + phosphate
-
 r
2.4.1.1 starch + alpha-D-glucose 1-phosphate preferred substrate Chlamydomonas reinhardtii starch + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme Chlamydomonas reinhardtii
2.4.1.1 dimer 2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 25 40 broad Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.5
-
 isoform PhoA Chlamydomonas reinhardtii
2.4.1.1 7.5
-
-
 Chlamydomonas reinhardtii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.2
-
 ADP-glucose presence of 1 mM orthophosphate, pH 7.0, 30°C Chlamydomonas reinhardtii
2.4.1.1 1
-
 ADP-glucose presence of 1 mM orthophosphate, pH 7.0, 30°C Chlamydomonas reinhardtii