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Literature summary extracted from

  • Andre, C.; Froehlich, J.E.; Moll, M.R.; Benning, C.
    A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis (2007), Plant Cell, 19, 2006-2022.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 6-phosphogluconate 192% of activity at 0.05 mM Arabidopsis thaliana
2.7.1.40 additional information not activated by 6-phosphogluconate Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.40 K314L no increased activity at pH 7.0 Arabidopsis thaliana
2.7.1.40 K325L no increased activity at pH 7.0 Arabidopsis thaliana
2.7.1.40 K344L no increased activity at pH 7.0 Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 AMP 1 mM, 77% of activity remaining Arabidopsis thaliana
2.7.1.40 ATP 1 mM, 77% of activity remaining; 1 mM, 87% of activity remaining Arabidopsis thaliana
2.7.1.40 glyoxylate 5 mM, 79% of activity remaining; 5 mM, 83% of activity remaining Arabidopsis thaliana
2.7.1.40 iso-citrate 10 mM, 70% of activity remaining; 10 mM, 75% of activity remaining Arabidopsis thaliana
2.7.1.40 L-glutamate 5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM; 5 mM, 59% of activity remaining, IC50: 6.2 mM Arabidopsis thaliana
2.7.1.40 additional information not inhibited by 6-phosphogluconate, dithiothreitol or sodium tetrathionate; not inhibited by AMP, dithiothreitol or sodium tetrathionate Arabidopsis thaliana
2.7.1.40 oxalate 0.2 mM, 50% of activity remaining; 0.2 mM, 71% of activity remaining, IC50: 0.41 mM Arabidopsis thaliana
2.7.1.40 oxaloacetate 2 mM, 82% of activity remaining; 2 mM, 88% of activity remaining Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.40 plastid
-
 Arabidopsis thaliana 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ strict requirement Arabidopsis thaliana
2.7.1.40 Mg2+ strict requirement Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 57000
-
 4 * 60000 + 4 * 57000, gel filtration Arabidopsis thaliana
2.7.1.40 60000
-
 4 * 60000 + 4 * 57000, gel filtration Arabidopsis thaliana
2.7.1.40 463000
-
 gel filtration Arabidopsis thaliana
2.7.1.40 476000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 Ni-NTA resin chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Arabidopsis thaliana ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 octamer 4 * 60000 + 4 * 57000, gel filtration Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 PK-alphabeta1
-
 Arabidopsis thaliana
2.7.1.40 PK-alphabeta2
-
 Arabidopsis thaliana
2.7.1.40 PKp
-
 Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.40 50 55 completely inactivated after 3 min of incubation at 50 to 55°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 8
-
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ADP pyruvate kinase activity is not observed in the absence of ADP Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.40 0.41
-
 0.2 mM, 71% of activity remaining, IC50: 0.41 mM Arabidopsis thaliana oxalate
2.7.1.40 6.2
-
 5 mM, 30% of activity remaining, IC50: 2.1 mM, IC50: 6.2 mM Arabidopsis thaliana L-glutamate
2.7.1.40 6.2
-
 5 mM, 59% of activity remaining, IC50: 6.2 mM Arabidopsis thaliana L-glutamate