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Literature summary extracted from

  • Isayenkova, J.; Wray, V.; Nimtz, M.; Strack, D.; Vogt, T.
    Cloning and functional characterization of two regioselective flavonoid glucosyltransferases from Beta vulgaris (2006), Phytochemistry, 67, 1598-1612.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.237 functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT71F1 Beta vulgaris
2.4.1.237 functionally expressed in Escherichia coli as His- and GST-tagged protein, UGT73A4 Beta vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.237 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid 0.01 mM, UGT71F1; 0.01 mM, UGT73A4 Beta vulgaris
2.4.1.237 Co2+ complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4 Beta vulgaris
2.4.1.237 Cu2+ complete inhibition near 0.04 mM, UGT71F1; complete inhibition near 0.04 mM, UGT73A4 Beta vulgaris
2.4.1.237 p-chloromercuribenzene sulfonic acid UGT71F1, 0.01mM, 22% inhibition; UGT73A4 activity is completely inhibited at 0.01mM Beta vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.237 0.0024
-
 quercetin UGT71F1 Beta vulgaris
2.4.1.237 0.011
-
 quercetin UGT73A4 Beta vulgaris
2.4.1.237 0.0192
-
 apigenin UGT73A4 Beta vulgaris
2.4.1.237 0.0268
-
 apigenin UGT71F1 Beta vulgaris
2.4.1.237 0.029
-
 betanidin UGT71F1 Beta vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.237 additional information no requirement for bivalent cations, UGT71F1 Beta vulgaris
2.4.1.237 additional information no requirement for bivalent cations, UGT73A4 Beta vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.237 54070
-
 calculated from sequence, UGT73A4 Beta vulgaris
2.4.1.237 54390
-
 calculated from sequence, UGT71F1 Beta vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.237 Beta vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.237 recombinant UGT71F1 Beta vulgaris
2.4.1.237 recombinant UGT73A4 Beta vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
2.4.1.237 UGT71F1 can not be frozen nor stored at 4°C as 50% activity is lost within 24 h Beta vulgaris
2.4.1.237 UGT73A4 can be stored at 4°C or frozen at -20°C or -80°C for several months without requiring addition of stabilizing agents such as bovine serum albumin or glycerol Beta vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.237 UDP-alpha-D-glucose + kaempferol 81% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + kaempferol 17% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + kaempferol 3-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin UGT71F1 Beta vulgaris UDP + quercetin 7-O-beta-D-glucoside + quercetin 3-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 Beta vulgaris UDP + quercetin 7-O-beta-D-glucoside + quercetin 4'-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + apigenin 40% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + apigenin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + apigenin 90% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + apigenin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + betanidin 28% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + betanidin 5-O-beta-D-glucoside + betanidin 6-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + caffeic acid 90% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + caffeoyl 3-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + chrysin 70% of the activity with quercetinUGT73A4 Beta vulgaris UDP + chrysin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + eriodictyol 48% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + eriodictyol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + esculetin 77% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + esculetin 6-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol-4'-methylether 42% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + kaempferol-4'-methylether 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + luteolin 17% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + luteolin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + luteolin as active as with quercetin, UGT73A4 Beta vulgaris UDP + luteolin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + myricetin 80% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 Beta vulgaris UDP + myricetin 4'-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + naringenin 10% of the activity with quercetin, UGT73A4 Beta vulgaris UDP + naringenin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + pinocembrin 25% of the activity with quercetinUGT73A4 Beta vulgaris UDP + pinocembrin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + pinocembrin 57% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + pinocembrin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + quercetagetin 84% of the activity with quercetin, UGT71F1 Beta vulgaris UDP + quercetagetin 3-O-beta-D-glucoside + quercetagetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + quercetagetin 28% of the activity with quercetin, 4'-OH glycosylation is preferred over 7-OH glycosylation, UGT73A4 Beta vulgaris UDP + quercetagetin 4'-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.237 monomer 1 * 54070, calculated from sequence, UGT73A4 Beta vulgaris
2.4.1.237 monomer 1 * 54390, calculated from sequence, UGT71F1 Beta vulgaris

Synonyms

EC Number Synonyms Comment Organism
2.4.1.237 UGT71F1
-
 Beta vulgaris
2.4.1.237 UGT73A4
-
 Beta vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.237 30
-
 UGT73A4 Beta vulgaris
2.4.1.237 37
-
 UGT71F1 Beta vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.237 6
-
 UGT73A4 Beta vulgaris
2.4.1.237 7 9 UGT71F1 Beta vulgaris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.237 Beta vulgaris calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT71F1
-
 5.6
2.4.1.237 Beta vulgaris calculated from sequence, MALDI-TOF-MS analysis with molecular masses of tags subtracted, UGT73A4
-
 5.8