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Literature summary extracted from

  • Wagemaker, M.J.; Eastwood, D.C.; van der Drift, C.; Jetten, M.S.; Burton, K.; Van Griensven, L.J.; Op den Camp, H.J.
    Argininosuccinate synthetase and argininosuccinate lyase: two ornithine cycle enzymes from Agaricus bisporus (2007), Mycol. Res., 111, 493-502.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.1 into the pGEM-T vector for sequence analysis Agaricus bisporus
6.3.4.5 cloned in Escherichia coli Agaricus bisporus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.5 additional information an alignment with ASS proteins from other organisms reveals high similarity with fungal (61–63%) and mammalian (51–55%) ASS proteins Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.1 52340
-
 the asl open reading frame encodes a protein of 464 amino acids with a calculated mass of 52337 Da Agaricus bisporus
6.3.4.5 47270
-
 deduced from cDNA Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.1 2-(Nomega-L-arginino)succinate Agaricus bisporus
-
 fumarate + L-arginine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.1 Agaricus bisporus Q9P870
-
-
6.3.4.5 Agaricus bisporus Q2Z0F6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.1 cap
-
 Agaricus bisporus
-
4.3.2.1 gill
-
 Agaricus bisporus
-
4.3.2.1 stipe
-
 Agaricus bisporus
-
6.3.4.5 cap Northern analyses of ass during fruiting body formation and post-harvest development reveals that expression is significantly up-regulated from developmental stage 3 on for all the tissues studied (gills, stip and cap). The expression reaches a maximum at the later stages of fruiting body growth, stages 6 and 7 Agaricus bisporus
-
6.3.4.5 gill Northern analyses of ass during fruiting body formation and post-harvest development reveals that expression is significantly up-regulated from developmental stage 3 on for all the tissues studied (gills, stip and cap). The expression reaches a maximum at the later stages of fruiting body growth, stages 6 and 7 Agaricus bisporus
-
6.3.4.5 additional information ASS is up-regulated within 3 h after harvest showing that the induction mechanism is very sensitive to the harvest event and emphasizes the importance of the arginine biosynthetic pathway/ornithine cycle in post-harvest physiology Agaricus bisporus
-
6.3.4.5 stipe Northern analyses of ass during fruiting body formation and post-harvest development reveals that expression is significantly up-regulated from developmental stage 3 on for all the tissues studied (gills, stip and cap). The expression reaches a maximum at the later stages of fruiting body growth, stages 6 and 7 Agaricus bisporus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.1 2-(Nomega-L-arginino)succinate
-
 Agaricus bisporus fumarate + L-arginine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.2.1 Argininosuccinate lyase
-
 Agaricus bisporus
4.3.2.1 ASL
-
 Agaricus bisporus
6.3.4.5 Argininosuccinate synthetase
-
 Agaricus bisporus
6.3.4.5 ASS
-
 Agaricus bisporus