Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vinge, L.E.; Andressen, K.W.; Attramadal, T.; Andersen, G.?.; Ahmed, M.S.; Peppel, K.; Koch, W.J.; Freedman, N.J.; Levy, F.O.; Skomedal, T.; Osnes, J.B.; Attramadal, H.
    Substrate specificities of G protein-coupled receptor kinase-2 and -3 at cardiac myocyte receptors provide basis for distinct roles in regulation of myocardial function (2007), Mol. Pharmacol., 72, 582-591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 expression of GRK2 and GRK3 in cardiac myocytes via adenoviral transfection, overview Rattus norvegicus
2.7.11.15 expression of minigene encoding peptide inhibitors of GRK2, the C-terminal fragments of GRK2, i.e. AdGRK2, and AdGRK2ct, using adenovirus infection of cardiac myocyte, with effects on rod-shaped morphology of cardiac myocytes, overview Rattus norvegicus
2.7.11.15 expression of minigene encoding peptide inhibitors of GRK3, the C-terminal fragments of GRK3, i.e. AdGRK3, or AdGRK3ct, using adenovirus infection of cardiac myocyte, with effects on rod-shaped morphology of cardiac myocytes, overview Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 additional information subcellular distribution analysis of GRK2 and GRK3 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.15 79000
-
 x * 79000, GRK3, SDS-PAGE, x * 81000, GRK2, SDS-PAGE Rattus norvegicus
2.7.11.15 81000
-
 x * 79000, GRK3, SDS-PAGE, x * 81000, GRK2, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + alpha1 beta-adrenergic receptor Rattus norvegicus substrate specificities of GRK2 and GRK3 in cardiac myocytes, overview ADP + phosphorylated alpha1 beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + alpha1-adrenergic receptor Rattus norvegicus
-
 ADP + alpha1-adrenergic receptor phosphate
-
 ?
2.7.11.15 ATP + beta1-adrenergic receptor Rattus norvegicus
-
 ADP + beta1-adrenergic receptor phosphate
-
 ?
2.7.11.15 ATP + rhodopsin Rattus norvegicus
-
 ADP + phosphorylated rhodopsin
-
 ?
2.7.11.15 additional information Rattus norvegicus GRK2 substrate specificity in adult rat cardiac myocytes, overview. GRK2 completely lacks the capacity to attenuate phenylephrine-stimulated IP generation ?
-
 ?
2.7.11.15 additional information Rattus norvegicus GRK3 substrate specificity in adult rat cardiac myocytes, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Rattus norvegicus
-
 male Wistar rats
-
2.7.11.15 Rattus norvegicus P26819 GRK2; male Wistar rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.15 cardiac myocyte
-
 Rattus norvegicus
-
2.7.11.15 cardiomyocyte
-
 Rattus norvegicus
-
2.7.11.15 heart
-
 Rattus norvegicus
-
2.7.11.15 additional information quantitative enzyme level determination Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.15 additional information
-
 quantitative enzyme level determination Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + alpha1 beta-adrenergic receptor
-
 Rattus norvegicus ADP + phosphorylated alpha1 beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + alpha1 beta-adrenergic receptor substrate specificities of GRK2 and GRK3 in cardiac myocytes, overview Rattus norvegicus ADP + phosphorylated alpha1 beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + alpha1-adrenergic receptor
-
 Rattus norvegicus ADP + alpha1-adrenergic receptor phosphate
-
 ?
2.7.11.15 ATP + beta1-adrenergic receptor
-
 Rattus norvegicus ADP + beta1-adrenergic receptor phosphate
-
 ?
2.7.11.15 ATP + rhodopsin
-
 Rattus norvegicus ADP + phosphorylated rhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin substrate in dark-adapted, urea-stripped rod outer segment membranes Rattus norvegicus ADP + phosphorylated rhodopsin
-
 ?
2.7.11.15 additional information GRK2 substrate specificity in adult rat cardiac myocytes, overview. GRK2 completely lacks the capacity to attenuate phenylephrine-stimulated IP generation Rattus norvegicus ?
-
 ?
2.7.11.15 additional information GRK3 substrate specificity in adult rat cardiac myocytes, overview Rattus norvegicus ?
-
 ?
2.7.11.15 additional information GRK2 substrate specificity, overview. GRK2 also shows activity with rhodopsin as substrate, cf. EC 2.7.11.14 Rattus norvegicus ?
-
 ?
2.7.11.15 additional information GRK3 substrate specificity, overview. GRK3 also shows activity with rhodopsin as substrate, cf. EC 2.7.11.14 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.15 ? x * 79000, GRK3, SDS-PAGE, x * 81000, GRK2, SDS-PAGE Rattus norvegicus
2.7.11.15 More GRK2 consists of three domains: an aminoterminal RGS homology domain, a central protein kinase domain, and a carboxyl-terminal pleckstrin homology, PH, domain Rattus norvegicus
2.7.11.15 More GRK3 consists of three domains: an aminoterminal RGS homology domain, a central protein kinase domain, and a carboxyl-terminal pleckstrin homology, PH, domain Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.15 G protein-coupled receptor kinase-2
-
 Rattus norvegicus
2.7.11.15 G protein-coupled receptor kinase-3
-
 Rattus norvegicus
2.7.11.15 GRK2
-
 Rattus norvegicus
2.7.11.15 GRK3
-
 Rattus norvegicus
2.7.11.15 More GRK2 and GRK3 constitute a subfamily among the different GRK isoforms Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 8
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Rattus norvegicus