EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.145 | medicine | mitochondrial targeting of Dm-dNK facilitates nucleoside and nucleoside analog phosphorylation and could be used as a strategy to enhance the efficacy of nucleoside analog phosphorylation and concomitantly their cytostatic potential | Drosophila melanogaster |
2.7.1.145 | medicine | mitochondrial targeting of multisubstrate nucleoside kinase facilitates nucleoside and nucleoside analog phosphorylation and could be used as a strategy to enhance the efficacy of nucleoside analog phosphorylation and concomitantly their cytostatic potential | Drosophila melanogaster |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.145 | expression in a thymidine kinase 1-deficient osteosarcoma cell line. Mitochondrial targeting of Dm-dNK facilitates nucleoside and nucleoside analog phosphorylation and could be used as a strategy to enhance the efficacy of nucleoside analog phosphorylation and concomitantly their cytostatic potential | Drosophila melanogaster |
2.7.1.145 | expression in osteosarcoma cell line | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.145 | additional information | engineering of multisubstrate nucleoside kinase targeted to the mitochondrial matrix and expression in thymidine kinase-1 deficient osteosarcoma cell line. Although the total deoxythymidine phosphorylation activity is similar in cells expressing multisubstrate nullceoside kinase in the nucleus or in the mitochondria, the cells expressing the enzyme in the mitochondria show higher sensitivity to the antiproliferative activity of pyrimidine nucleoside analogs, such as (E)-5-(2-bromovinyl)-2-deoxyuridine, 5-bromo-2-deoxyuridine, and 5-fluoro-2-deoxyuridine. Cells expressing the mitochondrial enzyme have an increased incorporation of [3H]dThd into DNA, due to a higher [3H]dTTP specific activity of the total dTTP pool in the cells in which the enzyme is targeted to the mitochondria | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.145 | mitochondrion | - |
Drosophila melanogaster | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | - |
- |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.145 | Dm-dNK | - |
Drosophila melanogaster |
2.7.1.145 | multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |