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Literature summary extracted from

  • Willmore, W.G.; Storey, K.B.
    Purification and properties of glutathione reductase from liver of the anoxia-tolerant turtle, Trachemys scripta elegans (2007), Mol. Cell. Biochem., 297, 139-149.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 glutathione inhibits the turtle liver GR only at high concentrations (6 mM), although this I50 value is within the physiological range of GSH concentrations in most cell types Trachemys scripta
1.8.1.7 KCl IC50: 0.51 M Trachemys scripta
1.8.1.7 additional information NADP+ does not inhibit turtle GR activity Trachemys scripta
1.8.1.7 Na2SO4 IC50: 0.45 M Trachemys scripta
1.8.1.7 NaCl IC50: 0.55 M Trachemys scripta
1.8.1.7 NH4Cl IC50: 0.62 M Trachemys scripta
1.8.1.7 Sodium acetate IC50: 0.77 M Trachemys scripta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.00446
-
 GSSG anoxic enzyme in 50 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM GSSG and 0.2 mM NADPH Trachemys scripta
1.8.1.7 0.00682
-
 NADPH anoxic enzyme in 50 mM potassium phosphate, pH 7.4, 1 mM EDTA, 0.1 mM GSSG and 0.2 mM NADPH Trachemys scripta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.7 KCl IC50: 0.51 M Trachemys scripta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 55000
-
 SDS-PAGE Trachemys scripta

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Trachemys scripta
-
 Trachemys scripta elegans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 ion exchange chromatography on hydroxylapatite, LKB 8101 isoelectrofocusing column chromatography, Sephacryl S-300 column gel filtration and Matrix Red dye ligand chromatography Trachemys scripta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 liver
-
 Trachemys scripta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 0.119
-
 crude supernatant of anoxic enzyme Trachemys scripta
1.8.1.7 0.418
-
 crude supernatant of aerobic enzyme Trachemys scripta
1.8.1.7 1.44
-
 anoxic enzyme after 109fold purification Trachemys scripta
1.8.1.7 5.5
-
 aerobic enzyme after 109fold purification Trachemys scripta

Storage Stability

EC Number Storage Stability Organism
1.8.1.7 4°C, pure enzyme without stabilizers such as glycerol, 8 days, 25% loss of activity Trachemys scripta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH + H+
-
 Trachemys scripta glutathione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Trachemys scripta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6.5
-
-
 Trachemys scripta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.7 4 10
-
 Trachemys scripta

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Trachemys scripta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.7 Trachemys scripta aerobic enzyme
-
 7.2
1.8.1.7 Trachemys scripta anoxic enzyme
-
 7.32

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.7 450
-
 IC50: 0.45 M Trachemys scripta Na2SO4
1.8.1.7 510
-
 IC50: 0.51 M Trachemys scripta KCl
1.8.1.7 550
-
 IC50: 0.55 M Trachemys scripta NaCl
1.8.1.7 620
-
 IC50: 0.62 M Trachemys scripta NH4Cl
1.8.1.7 770
-
 IC50: 0.77 M Trachemys scripta Sodium acetate