EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.99.4 | additional information | ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis | Mycolicibacterium smegmatis |
1.3.99.4 | additional information | the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.4 | into the pJam2 expression vector under the acetamidase promoter and introduced into the deltaksdD-1 mutant | Mycolicibacterium smegmatis |
1.3.99.4 | under control of the Phsp promoter and introduced into mutant cells of Mycobacterium smegmatis | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.99.4 | additional information | targeted disruption of the MSMEG5898 (ksdD-1) gene, but not the MSMEG4855 (ksdD-2) gene, results in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione, reversible by the introduction of the wild-type ksdD-1 gene into deltaksdD-1 or overexpression of ksdD-2 | Mycolicibacterium smegmatis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.4 | Mycobacterium tuberculosis | - |
- |
- |
1.3.99.4 | Mycobacterium tuberculosis H37Ra / ATCC 25177 | - |
- |
- |
1.3.99.4 | Mycolicibacterium smegmatis | - |
- |
- |
1.3.99.4 | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.99.4 | cholesterol + acceptor | KsdD2 exhibits low but detectable activity in the cholesterol degradation process | Mycolicibacterium smegmatis | ? | - |
? | |
1.3.99.4 | cholesterol + acceptor | KsdD2 exhibits low but detectable activity in the cholesterol degradation process | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.99.4 | 3-ketosteroid delta1-dehydrogenase | - |
Mycolicibacterium smegmatis |
1.3.99.4 | 3-ketosteroid delta1-dehydrogenase | - |
Mycobacterium tuberculosis |
1.3.99.4 | KsdD | - |
Mycobacterium tuberculosis |
1.3.99.4 | KsdD1 | - |
Mycolicibacterium smegmatis |
1.3.99.4 | KsdD2 | - |
Mycolicibacterium smegmatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.4 | FAD | - |
Mycolicibacterium smegmatis |