Literature summary extracted from

Leonard, E.; Yan, Y.; Koffas, M.A.
Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli (2006), Metab. Eng., 8, 172-181.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.11.9	expressed in Escherichia coli BL21Star and Rosetta(DE3) cells	Malus domestica
1.14.14.81	functional expression of the enzyme as fusion enzyme with a P450 reductase leading to biosynthesis of plant-specific di- and trihydroxylated flavonols in Escherichia coli strain BL21(DE3), feeding experiments and determination of the flavonoid spectra in different recombinant bacterial lines in vivo, overview	Catharanthus roseus
1.14.20.6	expressed in Escherichia coli strain BL21Star	Arabidopsis thaliana

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.11.9	Malus domestica	-	-	-
1.14.14.81	Catharanthus roseus	-	-	-
1.14.20.6	Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.11.9	(2S)-naringenin + 2-oxoglutarate + O2	-	Malus domestica	(2R,3R)-dihydrokaempferol + succinate + CO2	-	ir
1.14.11.9	eriodictyol + 2-oxoglutarate + O2	-	Malus domestica	(2R,3R)-dihydroquercetin + succinate + CO2	-	ir
1.14.20.6	(2S)-naringenin + 2-oxoglutarate + O2	-	Arabidopsis thaliana	(2R,3S)-cis-dihydrokaempferol + (2R,3R)-trans-dihydrokaempferol + apigenin + kaempferol + succinate + CO2 + H2O	-	?
1.14.20.6	dihydrokaempferol + 2-oxoglutarate + O2	-	Arabidopsis thaliana	kaempferol + succinate + CO2 + H2O	-	?
1.14.20.6	dihydromyricetin + 2-oxoglutarate + O2	-	Arabidopsis thaliana	myricetin + succinate + CO2 + H2O	-	?
1.14.20.6	dihydroquercetin + 2-oxoglutarate + O2	-	Arabidopsis thaliana	quercetin + succinate + CO2 + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.11.9	FHT	-	Malus domestica
1.14.11.9	flavanone 3beta-hydroxylase	-	Malus domestica
1.14.14.81	F3',5'H	-	Catharanthus roseus
1.14.14.81	plant P450 flavonoid 3',5'-hydroxylase	-	Catharanthus roseus
1.14.20.6	FLS	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)