EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.1.22 | additional information | two isoenzymes of 5alphaR is probably characteristic of the whole plant kingdom | Arabidopsis thaliana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.22 | into vector pTarget and expressed in COS-7 cells | Arabidopsis thaliana |
1.3.1.22 | into vector pTarget and expressed in COS-7 cells | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.1.22 | K204E | det2-1 mutant, substitution of lysine for Glu-204 totally inactivates the steroid 5alpha-reductase activity, residual activity for campestenone and progesterone | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.22 | 4-MA | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | AFA131 | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | AFA192 | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | AFA27 | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | AFA76 | active at 0.01 mM, most potent inhibitor among non-steroid compounds tested | Solanum lycopersicum | |
1.3.1.22 | AS601811 | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | finasteride | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | PD17 | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | PD91 | active at 0.01 mM | Solanum lycopersicum | |
1.3.1.22 | VG106 | active at 0.01 mM | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.22 | 0.00075 | - |
progesterone | - |
Solanum lycopersicum | |
1.3.1.22 | 0.02634 | - |
androstenedione | - |
Solanum lycopersicum | |
1.3.1.22 | 0.0446 | - |
testosterone | - |
Solanum lycopersicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.22 | Arabidopsis thaliana | - |
- |
- |
1.3.1.22 | Solanum lycopersicum | Q5K2N1 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.22 | callus | - |
Solanum lycopersicum | - |
1.3.1.22 | leaf | - |
Arabidopsis thaliana | - |
1.3.1.22 | leaf | higher levels | Solanum lycopersicum | - |
1.3.1.22 | root | - |
Solanum lycopersicum | - |
1.3.1.22 | seed | - |
Arabidopsis thaliana | - |
1.3.1.22 | seed | - |
Solanum lycopersicum | - |
1.3.1.22 | stem | - |
Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.22 | androstenedione + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-androstan-3,17-dione + NADP+ | - |
? | |
1.3.1.22 | campestenone + NADPH | reduction at very low levels | Solanum lycopersicum | 5alpha-campestan-3-one + NADP+ | - |
? | |
1.3.1.22 | additional information | no activity for campestenone | Arabidopsis thaliana | ? | - |
? | |
1.3.1.22 | progesterone + NADPH + H+ | - |
Arabidopsis thaliana | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
1.3.1.22 | progesterone + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
1.3.1.22 | testosterone + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-dihydrotestosterone + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.22 | 5alphaR | - |
Arabidopsis thaliana |
1.3.1.22 | 5alphaR | - |
Solanum lycopersicum |
1.3.1.22 | AtDET2 | - |
Arabidopsis thaliana |
1.3.1.22 | LeDET2 | - |
Solanum lycopersicum |
1.3.1.22 | steroid 5alpha-reductase | - |
Arabidopsis thaliana |
1.3.1.22 | steroid 5alpha-reductase | - |
Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.22 | NADPH | - |
Solanum lycopersicum |