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Literature summary extracted from

  • Ghaderi, D.; Strauss, H.M.; Reinke, S.; Cirak, S.; Reutter, W.; Lucka, L.; Hinderlich, S.
    Evidence for dynamic interplay of different oligomeric states of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by biophysical methods (2007), J. Mol. Biol., 369, 746-758.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.60 expressed in Sf-9 cells Rattus norvegicus
5.1.3.14 expression in insect cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.14 CMP-Neu5Ac allosterical feed-back-inhibition Rattus norvegicus
5.1.3.14 UDP competitive inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.60 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.60 83500
-
 4 * 83500, dynamic light scattering, the tetramer likely represents the active state of the enzyme in the cell Rattus norvegicus
2.7.1.60 85000
-
 4 * 85000, gel filtration Rattus norvegicus
2.7.1.60 334000
-
 dynamic light scattering Rattus norvegicus
2.7.1.60 340000
-
 gel filtration Rattus norvegicus
5.1.3.14 330000
-
 dynamic light-scattering Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.14 UDP-GlcNAc + H2O Rattus norvegicus biosynthesis of sialic acids ManNAc + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.60 Rattus norvegicus
-
-
-
5.1.3.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.60 MonoQ HR 5/5 column chromatography, PD-10 column chromatography, and Superdex 200 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.60 liver
-
 Rattus norvegicus
-
5.1.3.14 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.60 ATP + N-acetyl-D-mannosamine
-
 Rattus norvegicus ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
5.1.3.14 UDP-GlcNAc + H2O biosynthesis of sialic acids Rattus norvegicus ManNAc + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.60 tetramer 4 * 83500, dynamic light scattering, the tetramer likely represents the active state of the enzyme in the cell Rattus norvegicus
2.7.1.60 tetramer 4 * 85000, gel filtration Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.60 GNE
-
 Rattus norvegicus
2.7.1.60 UDP-GlcNAc-epimerase/ManNAc kinase
-
 Rattus norvegicus
2.7.1.60 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
-
 Rattus norvegicus
5.1.3.14 His-rGNE
-
 Rattus norvegicus
5.1.3.14 UDP-N-acetylglucosamine 2-epimerase
-
 Rattus norvegicus