Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sandrini, M.P.B.; Soederbom, F.; Mikkelsen, N.E.; Piskur, J.
    Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinases (2007), J. Mol. Biol., 369, 653-664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.76 expression in Escherichia coli Dictyostelium discoideum
2.7.1.113
-
 Dictyostelium discoideum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.76 three-dimensional structural model based on crystal structure of Mycoplasma mycoides deoxyadenosine kinase Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.76 0.004
-
 thymidine
-
 Dictyostelium discoideum
2.7.1.76 0.0227
-
 2'-deoxyadenosine
-
 Dictyostelium discoideum
2.7.1.76 0.0227
-
 deoxyadenosine
-
 Dictyostelium discoideum
2.7.1.76 0.146
-
 fludarabine
-
 Dictyostelium discoideum
2.7.1.76 0.146
-
 2'-fluoro-arabinosyl adenine
-
 Dictyostelium discoideum
2.7.1.113 0.0014
-
 deoxyguanosine
-
 Dictyostelium discoideum
2.7.1.113 0.206
-
 deoxyadenosine
-
 Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.76 28600
-
 x * 28600, calculated Dictyostelium discoideum
2.7.1.113 30000
-
 SDS-PAGE Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.76 ATP + deoxyadenosine Dictyostelium discoideum
-
 ADP + dAMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.76 Dictyostelium discoideum
-
-
-
2.7.1.76 Dictyostelium discoideum Q54YL2
-
-
2.7.1.113 Dictyostelium discoideum Q54UT2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.113 AX-4 cell
-
 Dictyostelium discoideum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.76 ATP + 2'-deoxyadenosine
-
 Dictyostelium discoideum ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.76 ATP + 2'-fluoro-arabinosyl adenine i.e. fludarabine Dictyostelium discoideum ADP + 2'-fluoro-arabinosyl adenine monophosphate
-
 ?
2.7.1.76 ATP + deoxyadenosine
-
 Dictyostelium discoideum ADP + dAMP
-
 ?
2.7.1.76 ATP + fludarabine
-
 Dictyostelium discoideum ADP + fludarabine monophosphate
-
 ?
2.7.1.76 ATP + thymidine
-
 Dictyostelium discoideum ADP + thymidine 5'-phosphate
-
 ?
2.7.1.76 CTP + 2'-deoxyadenosine CTP, 3% of the activity with ATP Dictyostelium discoideum CDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.76 GTP + 2'-deoxyadenosine GTP, 14% of the activity with ATP Dictyostelium discoideum GDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.76 additional information cannot efficiently phosphorylate any other native deoxyribonucleoside than deoxyadenosine Dictyostelium discoideum ?
-
 ?
2.7.1.76 additional information enzyme cannot effectively phopshorylate any other native deoxyribonucleodie besides 2'-deoxyadenosine Dictyostelium discoideum ?
-
 ?
2.7.1.76 UTP + 2'-deoxyadenosine UTP, 2% of the activity with ATP Dictyostelium discoideum UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.113 ATP + deoxyadenosine barely measurable phosphorylation of deoxyadenosine Dictyostelium discoideum ADP + dAMP
-
 ?
2.7.1.113 ATP + deoxyguanosine
-
 Dictyostelium discoideum ADP + dGMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.76 ? x * 28600, calculated Dictyostelium discoideum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.76 dAK
-
 Dictyostelium discoideum
2.7.1.113 DGK
-
 Dictyostelium discoideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.76 0.0081
-
 thymidine
-
 Dictyostelium discoideum
2.7.1.76 3.3
-
 fludarabine
-
 Dictyostelium discoideum
2.7.1.76 3.3
-
 2'-fluoro-arabinosyl adenine
-
 Dictyostelium discoideum
2.7.1.76 3.7
-
 2'-deoxyadenosine
-
 Dictyostelium discoideum
2.7.1.76 3.7
-
 deoxyadenosine
-
 Dictyostelium discoideum
2.7.1.76 8.1
-
 thymidine
-
 Dictyostelium discoideum
2.7.1.113 0.0047
-
 deoxyadenosine
-
 Dictyostelium discoideum
2.7.1.113 3
-
 deoxyguanosine
-
 Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.76 ATP
-
 Dictyostelium discoideum
2.7.1.113 ATP
-
 Dictyostelium discoideum