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Literature summary extracted from

  • Qian, C.; Wang, X.; Manzur, K.; Sachchidanand, K.; Farooq, A.; Zeng, L.; Wang, R.; Zhou, M.M.
    Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase (2006), J. Mol. Biol., 359, 86-96.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.356 analysis three-dimensional solution structure of vSET bound to S-adenosyl-L-homocysteine and a histone H3 peptide containing mono-methylated lysine 27, only invariant active site residue tyrosine 105 in vSET facilitates methyl transfer from S-adenosyl-L-homocysteine to the substrate lysine by aligning intermolecular interactions in the lysine access channel of the enzyme Paramecium bursaria Chlorella virus 1

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.356 F52Y can only mono- and di- but not tri-methylate H38(K27) Paramecium bursaria Chlorella virus 1
2.1.1.356 Y50F nearly abolishes di- and tri-methylation of H3(K27), but does not affect the mono-methylation Paramecium bursaria Chlorella virus 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.356 0.2762
-
 histone H3(K27) wild-type Paramecium bursaria Chlorella virus 1
2.1.1.356 0.4274
-
 histone H3(K27) mutant Y50F Paramecium bursaria Chlorella virus 1
2.1.1.356 3.9
-
 histone H3(K27) mutant F52Y Paramecium bursaria Chlorella virus 1

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.356 Paramecium bursaria Chlorella virus 1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.356 S-adenosyl-L-methionine + histone H3(K27)
-
 Paramecium bursaria Chlorella virus 1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.356 dimer in solution Paramecium bursaria Chlorella virus 1

Synonyms

EC Number Synonyms Comment Organism
2.1.1.356 histone H3 lysine 27 methyltransferase
-
 Paramecium bursaria Chlorella virus 1
2.1.1.356 SET domain histone lysine methyltransferase
-
 Paramecium bursaria Chlorella virus 1
2.1.1.356 vSET
-
 Paramecium bursaria Chlorella virus 1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.356 1.32
-
 histone H3(K27) wild-type Paramecium bursaria Chlorella virus 1
2.1.1.356 1.36
-
 histone H3(K27) mutant Y50F Paramecium bursaria Chlorella virus 1