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Literature summary extracted from

  • Faehnle, C.R.; Ohren, J.F.; Viola, R.E.
    A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschii (2005), J. Mol. Biol., 353, 1055-1068.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expression in Escherichia coli Methanocaldococcus jannaschii
1.2.1.11 into the pET41-41a vector for transformation into Rosetta-competent Escherichia coli cells Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.11 2.3 A resolution Methanocaldococcus jannaschii
1.2.1.11 the structure of aspartate-beta-semialdehyde dehydrogenase has been determined to 2.3 A resolution using multiwavelength anomalous diffraction phasing of a seleno-methionine-substituted derivative Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.11 2'-phosphoribose AMP
-
 Methanocaldococcus jannaschii
1.2.1.11 ADP
-
 Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.11 0.013
-
 NADP+
-
 Methanocaldococcus jannaschii
1.2.1.11 1.7
-
 NAD+
-
 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Methanocaldococcus jannaschii
-
 L-4-aspartyl phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11 anion exchange chromatography and gel-filtration Methanocaldococcus jannaschii
1.2.1.11 using anion-exchange chromatography, Source 15Q, followed by gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NAD+
-
 Methanocaldococcus jannaschii L-4-aspartyl phosphate + NADH + H+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Methanocaldococcus jannaschii L-4-aspartyl phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.11 homodimer crystallization Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Methanocaldococcus jannaschii
1.2.1.11 aspartate-beta-semialdehyde dehydrogenase
-
 Methanocaldococcus jannaschii
1.2.1.11 aspartate-semialdehyde dehydrogenase
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Methanocaldococcus jannaschii
1.2.1.11 NADPH
-
 Methanocaldococcus jannaschii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.11 0.05
-
 2'-phosphoribose AMP
-
 Methanocaldococcus jannaschii
1.2.1.11 0.4
-
 ADP
-
 Methanocaldococcus jannaschii